Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260112213310_lt0i ATAGATGGACGTCGCATCCGCGTCGACTACTCGATCACGCACCGTGCGCATACC(/T)CGACACCCGGTATCTACATGGGGAAACCTACGT NGG 10 8 17 8 24 0 100 5 10
    260112211125_5njk AGACTGGTCGTTCTCGAGGATTTTGTTTCGTGTACTTCGAGAACCAGGAGGATGCGAAAGTTGCAAAGAACGAGTGTACAGGCATGGAGATAGATGGACGTCGCATCCGCGTCGACTACTCGATCACGCACCGTGCGCATACCC(/T)CGACACCCGGTATCTACATGGGGAAACCTACG NGG 10 8 17 8 24 0 100 5 10
    260112204505_91uk GGAGATAGATGGACGTCGCATCCGCGTCGACTACTCGATCACGCAgCGTGCGCATACC(/T)CGACACCCGGTATCTACATGGGGAAACCTACGTGAGTATTAAGCTCAATTTAGTAATTTCAG NGG 10 8 17 8 24 0 100 5 10
    260112154729_9xd0 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260104112011_v0z9 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260103225750_ijgb CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260102161223_o4m3 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251231154913_bc7q CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251227160907_panw CTGGATGTACTGGAATATATACATGAAAATGAGTATGTTCATGGTGA(/C)TATAAAAGCCGCAAATCTACTGTTGGATTTTACAAATCCAGACCGG NGG 10 8 17 8 24 0 100 5 10
    251227160514_8299 CTGGATGTACTGGAATATATACATGAAAATGAGTATGTTCATGGTGA(/C)TATAAAAGCCGCAAATCTACTGTTGGATTTTACAAATCCAGACCGG NGG 10 8 17 8 24 0 100 5 10