Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    251027234115_6ze5 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATTTT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251027234106_it7d CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATTT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251027233918_4ws7 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251026165401_m765 CCCTGTCTCTCTCTCTCTCGTACGGAGGGGGGTCGGCGCGCGCGAATCGAATCGCTCCCGGTTTCTCTCTCTCTCTCTCTCTCTCTGTGGT(-TCCAC)CCGCCGGGTTCTCATCCGCCGTGTGCGCCAGCAGGAGTCAGTTTGGGGGTGGTTATCAGTTCGGACAGGGTCCCGGGTGTCTGTAC NGG 10 8 17 8 24 0 100 5 10
    251026164926_80r5 CCCTGTCTCTCTGTTCCGTACGGAGGCAGACCGGGTCGGTACGCGCTCGAATCGAATCGCTCCCGGTTTCGTCCTCTCTCTCTCATTACTGGGTCGGT(-TCCAC)CCGCCGGGTTCTCATCCGCCGTGTGCGCCAGCAGGAGTCAGTTTGGGGGTGGTTATCAGTTCGGACAGGGTCCCGGGTGTCTGTAC NGG 10 8 17 8 24 0 100 5 10
    251023035522_qv4j CTGGACCGCTACCTCGCCATCACGTCGCCCTTTCGCTACCAGAGTTTGCTGACGCGCGCGCGAGCGCGGGCCCTCGTGTGCACAGTGTGGGCCATCTCGGC(/G)GTTGGTGTCCTTCCTGCCCATCCTCATGCACTGGTGGCGGGCCGAGAGCGACGAAGCGCGCCGCTGCTACAACGACCCCAAGTGCTGCGATTTCGTCACC NGG 10 8 17 8 40 0 100 5 10
    251021165841_htas TCCTTGTGTCTTTCTGTTTGTCCCCACAGTCCCCAGGGAAAAGCCTTTCGCTCTAAAGTGGAGTTGATTGCGTACTTCGAAAAGGTAGGCGACACATCCCTGGACCCTAATGATTTTGACTTCACGGTAACTGGGAGAGGGAGCCCCTCCCGGC(/T)GAGAGCAGAAACCACCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGAGGCCGGGGACGCCCCAAAGGGAGCGGCACCACGAGACCCAAGGCGGCCACGTCAGAGGGTGTGCAGGTGAAAAGGGTCCTGGAGAAAAGTCCT NGG 10 8 17 8 24 0 100 5 10
    251021164002_6ais GTTCCTTGTGTCTTTCTGTTTGTCCCCACAGTCCCCAGGGAAAAGCCTTTCGCTCTAAAGTGGAGTTGATTGCGTACTTCGAAAAGGTAGGCGACACATCCCTGGACCCTAATGATTTTGACTTCACGGTAACTGGGAGAGGGAGCCCCTCCCGGC(/T)GAGAGCAGAAACCACCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGAGGCCGGGGACGCCCCAAAGGGAGCGGCACCACGAGACCCAAGGCGGCCACGTCAGAGGGTGTGCAGGTGAAAAGGGTCCTGGAGAAAAGTCCTGGGAAGCTC NGG 10 8 17 8 24 0 100 5 10
    251021163955_ovbq GTTCCTTGTGTCTTTCTGTTTGTCCCCACAGTCCCCAGGGAAAAGCCTTTCGCTCTAAAGTGGAGTTGATTGCGTACTTCGAAAAGGTAGGCGACACATCCCTGGACCCTAATGATTTTGACTTCACGGTAACTGGGAGAGGGAGCCCCTCCCGGC(/T)GAGAGCAGAAACCACCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGAGGCCGGGGACGCCCCAAAGGGAGCGGCACCACGAGACCCAAGGCGGCCACGTCAGAGGGTGTGCAGGTGAAAAGGGTCCTGGAGAAAAGTCCTGGGAAGCTC NGG 10 8 17 8 24 0 100 5 10
    251021153045_nf8w TCCTTGTGTCTTTCTGTTTGTCCCCACAGTCCCCAGGGAAAAGCCTTTCGCTCTAAAGTGGAGTTGATTGCGTACTTCGAAAAGGTAGGCGACACATCCCTGGACCCTAATGATTTTGACTTCACGGTAACTGGGAGAGGGAGCCCCTCCCGGC(/T)GAGAGCAGAAACCACCTAAGAAGCCCAAATCTCCCAAAGCTCCAGGAACTGGCAGAGGCCGGGGACGCCCCAAAGGGAGCGGCACCACGAGACCCAAGGCGGCCACGTCAGAGGGTGTGCAGGTGAAAAGGGTCCTGGAGAAAAGTCCT NGG 10 8 17 8 24 0 100 5 10