Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250911084008_qln9 GATTCTATTCCCCAGTTAGAAAGACTGCAAACTGGTACTTGAAAACTCTGCGTTTGTCCTGTCATTCCATTTGTAGTTAAGGGTTTCACCATATGACATCCTCGGTGTTAACCAGAGGCCTTGGTGCTGATTCACAAGAACCTAAGCTGTCACTGAAATGAACTGAGAAAGTATCAGGTGGGGGGGGGGGGGAGAAGACAGCATGTTTCCATAGGAAAATGGACCAAAAGTGCAAGTTTTGGTTCAACATTATTGAAA(+ctt)TCCTACAGGTCATTGCTGAACGAGTCAAAGAAAGGAAGGCAGAGGAAGACTGGACGGGTGCTGGCAGGGGTCCTATCCCCTCCAAAAATAAGCGCAAGGCTTTCCTTGACTTGCTTTTGAGTGTGACTGATGAGGAAGGAAACAGATTAAGCCAGGAAGACATCCGAGAGGAAGTTGACACCTTCATGTTTGAGGTAACTTTTGTCGCCAGGTAATTTTCCT NGG 10 8 17 8 24 0 100 5 10
    250911083947_edrv GATTCTATTCCCCAGTTAGAAAGACTGCAAACTGGTACTTGAAAACTCTGCGTTTGTCCTGTCATTCCATTTGTAGTTAAGGGTTTCACCATATGACATCCTCGGTGTTAACCAGAGGCCTTGGTGCTGATTCACAAGAACCTAAGCTGTCACTGAAATGAACTGAGAAAGTATCAGGTGGGGGGGGGGGGGAGAAGACAGCATGTTTCCATAGGAAAATGGACCAAAAGTGCAAGTTTTGGTTCAACATTATTGAAATCCTAC(+ctt)AGGTCATTGCTGAACGAGTCAAAGAAAGGAAGGCAGAGGAAGACTGGACGGGTGCTGGCAGGGGTCCTATCCCCTCCAAAAATAAGCGCAAGGCTTTCCTTGACTTGCTTTTGAGTGTGACTGATGAGGAAGGAAACAGATTAAGCCAGGAAGACATCCGAGAGGAAGTTGACACCTTCATGTTTGAGGTAACTTTTGTCGCCAGGTAATTTTCCT NGG 10 8 17 8 24 0 100 5 10
    250911083912_uq4s GATTCTATTCCCCAGTTAGAAAGACTGCAAACTGGTACTTGAAAACTCTGCGTTTGTCCTGTCATTCCATTTGTAGTTAAGGGTTTCACCATATGACATCCTCGGTGTTAACCAGAGGCCTTGGTGCTGATTCACAAGAACCTAAGCTGTCACTGAAATGAACTGAGAAAGTATCAGGTGGGGGGGGGGGGGAGAAGACAGCATGTTTCCATAGGAAAATGGACCAAAAGTGCAAGTTTTGGTTCAACATTATTGAAATCC(+ctt)TACAGGTCATTGCTGAACGAGTCAAAGAAAGGAAGGCAGAGGAAGACTGGACGGGTGCTGGCAGGGGTCCTATCCCCTCCAAAAATAAGCGCAAGGCTTTCCTTGACTTGCTTTTGAGTGTGACTGATGAGGAAGGAAACAGATTAAGCCAGGAAGACATCCGAGAGGAAGTTGACACCTTCATGTTTGAGGTAACTTTTGTCGCCAGGTAATTTTCCT NGG 10 8 17 8 24 0 100 5 10
    250911083814_i29q AACTGAGAAAGTATCAGGTGGGGGGGGGGGGGAGAAGACAGCATGTTTCCATAGGAAAATGGACCAAAAGTGCAAGTTTTGGTTCAACATTATTGAAATCC(+ctt)TACAGGTCATTGCTGAACGAGTCAAAGAAAGGAAGGCAGAGGAAGACTGGACGGGTGCTGGCAGGGGTCCTATCCCCTCCAAAAATAAGCGCAAGGCTTTCCTTGACTTGCTTTTGAGTGTGACTGATGAGGAAGGAAACAGATTAAGCCAGGAAGACATCCGAGAGGAAGTTGACACCTTCATGTTTGAGGTAACTTTTGTCGCCAGGTAATTTTCCT NGG 10 8 17 8 24 0 100 5 10
    250910123824_gqsf gaccaggcctgagcaacaaagcgagatcccatctctacaaaaaccaaaaagttaaaaatcagctgggtacggtggcacgtgcctgtgatcccagctacttgggaggctgaggcaggag(-gatcgcctgagcccaggaggtggaggttgcagtgagccatgatcgagccactgcactccagcctgggcaacagatgaagaccctatttcagaaatacaactataaaaaaataaataaatcctccagtctggatcgtttgacgggacttcaggttctttctgaaatcgccgtgttactgttgcactgatgtccggagagacagtgacagcctccgtcagactcccgcgtgaagatgtcacaagggattggcaattgtccccagggacaaaacactgtgtcccccccagtgcagggaaccgtgataagcctttctggtttcggagcacgtaaatgcgtccctgtacagatagtggggattttttgttatgtttgcactttgtatattggttgaaactgttatcacttatatatatatatatacacacatatatataaaatctatttatttttgcaaaccctggttgctgtatttgttcagtgactattctcggggccctgtgtagggggttattgcctctgaaatgcctcttctttatgtacaaagattatttgcacgaactggactgtgtgcaacgctttttgggagaatgatgtccccgttgtatgtatgagtggcttctgggagatgggtgtcactttttaaaccactgtatagaaggtttttgtagcctgaatgtcttactgtgatcaattaaatttcttaaatgaaccaatttgtctaaactcgatgcacgttcttctgttcgcgcgcttctttttgtttttttttttttcctgagatggagcctggctctgtcacccctggctggagtgcagtggcatgatctcggcttactgcaagctccgcctcccaggttcaagcaattctcctgcctcagcctccctagtagctaggattacaggtgagtgccaccacgcctggccaattttttttttttttttttttttgagacagagtctcgctctgtcacccaggctggagtgcagtggtgtgatctcggctcactgcaagctctgcctcccaggttaatgccattctcctgtctcagcctcctgagtagctggggccacaggcgcctgccaccacgcccggctaatttttttttgtacttcttttagtacagacggggtttcaccatgttagccaggatggtctcgatctcctgaccttgtgatccacctgcttcggcctcccaaagtgctgagattacaggcgtgagccaccgcgggtggccaacgctaatttttttgtttttttagatggagtcttgctctgtcgcccaggctggagtgcagtggcgtgatctctgcctactgcaagctccgcctcccgggttcatgccattctcctgcctcagcctcctgagtaactgggactacaggcacccgccaccacgcccggctaattttttgtatttttagtagagacagggtttcaccgtgttagccaggatggtcttgatctcctgaccttgtgatccacccgtctcggcctcccaaagtgctgggattagaggtgtgagccaccacacctggcctagcctggctaatttttgtatttttggtagagacggggtttcaccatgttggtcaggctggtcttgaacttctgacctcaggtaatctgcctgcctcagtctcccaaagtgctgggattacaggtgtgagccaccgcgcctggcctcacttccttctgtcatctgtttgtggattggactccccaggagaaggacccagaaggggaagactcccagaactccgggcaagatgcaatctccgtgggctgccacagtgcctggcaggtgctgtgatggctgagctggtgattgtgttctctgctgtcgcttctctgagttggagattttgtcaagtcccctgctcatccattcatacactcgacaaatatctgttgagtgctaagtgcgaaccatgctctgccgtaggcttgtgggacactacaggggatataagaaatgaaagccgggtgtggtggctcacacctgtaatcctagcagtttgggaggccgaggcgggcagatcatgaggtcaggagatcgagaccatcctagctaacacagtgaaaccccatctctactaaaaatacaaaaaattagccaggcgtggtggtgggcgcctgtagtcccagctgcttgggaggctgaggcaggagaatagcgtgaacctgggagttggagcttgcagtgagccgagatcgcaccactgcactctagcctgggcaacagagcaagactccatctacaaaaaaaaaaaaaagaaatgaagtcttgatacggtggctcatgcctgtaatcccagcactttgggaggccaaggcaggcggatcacgagctcaggagatcgagaccatcctggccaacgtggcgaaacccagtctctactaaagatacaaaaaattagccaggcatggtggcgggcgcctgtagtcccagctacttgggaggctgaggcaggagaatggcatgaa)cccaggaggcagagcttgcagtgagctgagatcgcgccactgcactccagcctgggcgacagagcgagactctgtctcaaaaaaaaaacaaaaaagaaatgaaaatctccctgtctgtccttaaggacctgacatcattttattttatttatttttttgagacaaggtcttgctttattgcccaggctggagtgcagtggcacaatcttggctcactgcaacctccacctcccgggt NGG 10 8 17 8 24 0 100 5 10
    250908164152_fvp4 ggcaacatagtaagaccccatctcttaaaaaatgaatttggccagacacaggtgcctcacgcctgtaatcccagcactttgggaggctgagctggatcacttgagttcaggagttggagaccaggcctgagcaacaaagcgagatcccatctctacaaaaaccaaaaagttaaaaatcagctgggtacggtggcacgtgcctgtgatcccagctacttgggaggctgaggcaggaggatcgcctgagcccaggag(-gtggaggttgcagtgagccatgatcgagccactgcactccagcctgggcaacagatgaagaccctatttcagaaatacaactataaaaaaataaataaatcctccagtctggatcgtttgacgggacttcaggttctttctgaaatcgccgtgttactgttgcactgatgtccggagagacagtgacagcctccgtcagactcccgcgtgaagatgtcacaagggattggcaattgtccccagggacaaaacactgtgtcccccccagtgcagggaaccgtgataagcctttctggtttcggagcacgtaaatgcgtccctgtacagatagtggggattttttgttatgtttgcactttgtatattggttgaaactgttatcacttatatatatatatatacacacatatatataaaatctatttatttttgcaaaccctggttgctgtatttgttcagtgactattctcggggccctgtgtagggggttattgcctctgaaatgcctcttctttatgtacaaagattatttgcacgaactggactgtgtgcaacgctttttgggagaatgatgtccccgttgtatgtatgagtggcttctgggagatgggtgtcactttttaaaccactgtatagaaggtttttgtagcctgaatgtcttactgtgatcaattaaatttcttaaatgaaccaatttgtctaaactcgatgcacgttcttctgttcgcgcgcttctttttgtttttttttttttcctgagatggagcctggctctgtcacccctggctggagtgcagtggcatgatctcggcttactgcaagctccgcctcccaggttcaagcaattctcctgcctcagcctccctagtagctaggattacaggtgagtgccaccacgcctggccaattttttttttttttttttttttgagacagagtctcgctctgtcacccaggctggagtgcagtggtgtgatctcggctcactgcaagctctgcctcccaggttaatgccattctcctgtctcagcctcctgagtagctggggccacaggcgcctgccaccacgcccggctaatttttttttgtacttcttttagtacagacggggtttcaccatgttagccaggatggtctcgatctcctgaccttgtgatccacctgcttcggcctcccaaagtgctgagattacaggcgtgagccaccgcgggtggccaacgctaatttttttgtttttttagatggagtcttgctctgtcgcccaggctggagtgcagtggcgtgatctctgcctactgcaagctccgcctcccgggttcatgccattctcctgcctcagcctcctgagtaactgggactacaggcacccgccaccacgcccggctaattttttgtatttttagtagagacagggtttcaccgtgttagccaggatggtcttgatctcctgaccttgtgatccacccgtctcggcctcccaaagtgctgggattagaggtgtgagccaccacacctggcctagcctggctaatttttgtatttttggtagagacggggtttcaccatgttggtcaggctggtcttgaacttctgacctcaggtaatctgcctgcctcagtctcccaaagtgctgggattacaggtgtgagccaccgcgcctggcctcacttccttctgtcatctgtttgtggattggactccccaggagaaggacccagaaggggaagactcccagaactccgggcaagatgcaatctccgtgggctgccacagtgcctggcaggtgctgtgatggctgagctggtgattgtgttctctgctgtcgcttctctgagttggagattttgtcaagtcccctgctcatccattcatacactcgacaaatatctgttgagtgctaagtgcgaaccatgctctgccgtaggcttgtgggacactacaggggatataagaaatgaaagccgggtgtggtggctcacacctgtaatcctagcagtttgggaggccgaggcgggcagatcatgaggtcaggagatcgagaccatcctagctaacacagtgaaaccccatctctactaaaaatacaaaaaattagccaggcgtggtggtgggcgcctgtagtcccagctgcttgggaggctgaggcaggagaatagcgtgaacctgggagttggagcttgcagtgagccgagatcgcaccactgcactctagcctgggcaacagagcaagactccatctacaaaaaaaaaaaaaagaaatgaagtcttgatacggtggctcatgcctgtaatcccagcactttgggaggccaaggcaggcggatcacgagctcaggagatcgagaccatcctggccaacgtggcgaaacccagtctctactaaagatacaaaaaattagccaggcatggtggcgggcgcctgtagtcccagctacttgggaggctgaggcaggagaatggcatgaacccaggag)gcagagcttgcagtgagctgagatcgcgccactgcactccagcctgggcgacagagcgagactctgtctcaaaaaaaaaacaaaaaagaaatgaaaatctccctgtctgtccttaag NGG 10 8 17 8 24 0 100 5 10
    250908090803_gowyztk9 AACATGTCCCAAATTTCTAAAATTCTGCTTCTCTGCCAAAAGCAATGTCTTTCTTGGTTGATATTTGAGTTTTAAAAGGGTCAAATCTTTCTAATTTTTTG(/T)TATCTTTAGAGGGCAGCACTAGAAGAAATCAGCAGGTCTAATCCCACCAGTAAGAAAACTACCACTTCTTGATTTTTACAGATTTAAAAAAATCTTTTCAG NG 10 8 17 8 24 0 100 5 10
    250908090803_gowyyu1w AATATTCGTTGGGTTTCATGTTAAGAAGCCTGTGGTCTAGGAGTGCTATTCAGTGTTTCTTTTCCTGATAAACACTTTGAATATTTTTTTTGTGTTTTTGT(/C)TTCCTTTTCTGAAGCTGTTCCTCCTTTTAAATATTTTTAATCACATTGATAAAATCTATCCTTCACCACCTCTGGTTCTACTATAGTTGATTTTTATTTTA NG 10 8 17 8 24 0 100 5 10
    250908090803_gowyysob CAAAAGATATCCCTGTTTTCCTAATAATGTAAACAGTAAAACTGAGGGTTTCAGTTAAAGGGATAATTCGTAACAAAATCCCATTGTTGAAACAATGGTCA(/C)AACATAAACATCTTATAATTCAGATCTGCATTTGGTAACAGGAGAATTGAGAGTTTTGATGCTTTGCTGGTTTTCCCACAGTGGGAGATGTAGATACCATT NG 10 8 17 8 24 0 100 5 10
    250908090803_gowyxk8k GTCAACAAACTGTTCGTTTTTAAGTTACAAATTTGAATTTAATGTTGTCATCATCGTCATGTGTTTCCCCAAAGGGAAGCCAGTCATTGACCATTTAAAAA(/C)GTCTCCTGCTAAGTATGGAAATCAGACAGTAAGAGAAAGCCAAAAAGCAATGCAGAGAAAGGTGTCCAAGCTGTCTTCAGCCTTCCCCAGCTAAAGAGCAG NG 10 8 17 8 24 0 100 5 10