Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260707233402_pi6e TGGTGAAAAGGAAACTGCCATCCTTACCCCGTTTTCAGGCTGACCAAGGCG(/A)TCCTCTACTCCTTTCTGATTAAATTTGGTCATGTACTGATGCATGTAGGGATAGTTATTCCGAATGTTTCTCTCCGTGCTTCCATTAGGCACTGTCCCAAATCGAAAAGGTGGGGAATAGTCATGAGGTCTCTGAAACTGGAGAGAGAAC NGG 10 12 14 10 20 0 100 5 10
    260707230739_6bdn ctgccatccttaccccgttttcaggctgaccaaggcgtcctctactcctttctgattaaatttggtcatgtactgatgcatgtaggg(/a)atagttattccgaatgtttctctccgtgcttccattaggcactgtcccaaatcgaaaaggtggggaatagtcatgaggtctctgaaactggagagagaacgagaaaggaagaga NGG 10 12 14 10 20 0 100 5 10
    260707230437_bjel ctgccatccttaccccgttttcaggctgaccaaggcgtcctctactcctttctgattaaatttggtcatgtactgatgcatgtaggg(/a)atagttattccgaatgtttctctccgtgcttccattaggcactgtcccaaatcgaaaaggtggggaatagtcatgaggtctctgaaactggagagagaacgagaaaggaagaga NGG 10 8 17 8 24 0 100 5 10
    260707220013_ux6k TGGGATTGTGCTGAACGTACAGCAAGGCCAAACGGTTAGACCAATTACACTAGTTCCAGCCCCAGGTACCCAGTTTGTTAAGCCGACAGTTGGAGTTCCACAAGTGTTCTCCCAGATGACCCCTGTGAGGCCAGGCTCCACAATGCCTGTGAGGCCCACCACCAACACCTTCACCACCGTCATCCCGGCCACTCTTACCATTCGAAGCACCGTCCCACAGTCCCAGTCCCAGCAGACCAAGTCCACTCCCAGCACTTCTACCACTCCCAC(/T)TGCCACACAGCCAACCTCACTGGGGCAACTAGCTGTTCAGTCTCCAGGCCAGTCAAACCAGACCACGAATCCCAAGCTAGCTCCCTCCTTCCCCTCTCCACCTGCAGTGAGCATTGCCAGCTTTGTCACTGTGAAGCGACCTGGTGTTACAGGCGAAAATAGCAATGAAGTGGCCAAATTGGTGAATACCCTTAACACCATCCCTTCCCTGGGCCAGAGTCCTGGGCCAGTGGTGGTGTCCAACAACAGCTCTGCTCATGGCTCTCAAAGAACCAGCGGACCTGAGTCTTCAATGAAAGTGACCTCTTCCATCCCAGTATTTGACCTCCA NGG 10 8 17 8 24 0 100 5 10
    260707215924_kf10 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260707215017_g9r0 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260707214445_qjrw TGGTGAAAAGGAAACTGCCATCCTTACCCCGTTTTCAGGCTGACCAAGGCGTCCTCTACTCCTTTCTGATTAAATTTGGTCATGTACTGATGCATGTAGGG(/A)ATAGTTATTCCGAATGTTTCTCTCCGTGCTTCCATTAGGCACTGTCCCAAATCGAAAAGGTGGGGAATAGTCATGAGGTCTCTGAAACTGGAGAGAGAACGAGAAAGGAAGAGAGAGAGTAGGAAAAGAAAGAGAAGGGAGGAGGGGGA NGG 10 12 15 8 24 0 80 5 10
    260707213612_ku6a TAAACTCAAAGATCTTGCTTTTCATAGACATCTCTCAGCTCTGGATAAATAATATAAACAAGGTTAAAATCTGAACATTTGGAAGTATGAGAATGTAATTTGTACTTTGCAGATTGTAAATGCCTTCTGCCAGGAAACATCACCTGAGATGAAGGGGAAGGTGCTCACTCG(/A)GTTAAAGCACATTGTAGAATTGCAAATAATCCTGGAAAAGTACTTGGCAGGTAAGAGAAGTGTCCTATACTGGTAGTACTACTAGGCCAGTAGTGAGGCAATAAAGCACTTAGCTTTCCCTGATACCAAAACCAGACAAAGACACATCAAAAAAAGAAATCTCAGGCCAA NGG 10 8 17 8 24 0 100 5 10
    260707150032_g9a3 CATTTAAGCAAAATCCTTCTTTGGCAAAGTCTAGTTTTAAAAGTAAACTGCTTGCCAAACAACAATTTGTTAGGAATTCTTGGGGGAATTGGTTTCTAATATTGGTGTGGGGCTGAACCCTCAG(+actcagctac)CCAATACGTTGATCCATTATTATCTGAAGGGGGGGGGGGCTTGAAATCCAAGAATATTGGAATCTGTTTCTTAAAGTGACATATTAGTTAGAAAACATCTGTGAACATTCTTC NGG 10 8 17 8 24 0 100 5 10
    260707145941_boz7 CATTTAAGCAAAATCCTTCTTTGGCAAAGTCTAGTTTTAAAAGTAAACTGCTTGCCAAACAACAATTTGTTAGGAATTCT(+actggacac)TGGGGGAATTGGTTTCTAATATTGGTGTGGGGCTGAACCCTCAGCCAATACGTTGATCCATTATTATCTGAAGGGGGGGGGGGCTTGAAATCCAAGAATATTGGAATCTGTTTCTTAAAGTGACATATTAGTTAGAAAACATCTGTGAACATTCTTC NGG 10 8 17 8 24 0 100 5 10