Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250421184232_rluz CACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGGCAGAGGACCCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCT(+aag)AGCCTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGG NGG 10 8 17 8 24 0 100 5 10
    250421184218_nnom CACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGGCAGAGGACCCAAGTTCAATTTCCAGCATACACCAGGC(+aag)AGTAGTCACAACTACCCATAAGCTCCACCTAGCCTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGG NGG 10 8 17 8 24 0 100 5 10
    250421184121_yzk6 CACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGG(+ctt)CAGAGGACCCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCTAGCCTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGGGTAAGAGCAGTGTATTGCTCTTGAGGGGACCTGGGTCCAGGCACACACTTGGCACACATACATACATGAAGACAAGACACTCAGAAGTAAATCTATAAAGGGGGTCACCTATTTTTATTTTCTGTGGGTATTCATGTCTCAGTGCATTGTGGTGCCAGGC NGG 10 8 17 8 24 0 100 5 10
    250421184059_zqco TTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAGCACTC(+ctt)CAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGGCAGAGGACCCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCTAGCCTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGG NGG 10 8 17 8 24 0 100 5 10
    250421184039_dmbm GATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAGCACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACA(+aag)TAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGGCAGAGGACCCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCTAGCCTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGG NGG 10 8 17 8 24 0 100 5 10
    250421183944_p767 ATTTTTGTTAGGATTTGTTTCTAAATGAGGGATTTTTTGGTGTTTTGTTTTACATATATAAGTGTGTACCATATGTATCTAGTGCCCAATGATCAGAAGAGGGCGTTGGGTCTCTTGATAATTATGAGCCACCATGGGGGCATTGGAAACCAAATCCATGTTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCT(+aag)CTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421183902_ohkl ATTTTTGTTAGGATTTGTTTCTAAATGAGGGATTTTTTGGTGTTTTGTTTTACATATATAAGTGTGTACCATATGTATCTAGTGCCCAATGATCAGAAGAGGGCGTTGGGTCTCTTGATAATTATGAGCCACCATGGGGGCATTGGAAACCAAATCCATGTTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGT(+ctt)GCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421183901_q3t6 ATTTTTGTTAGGATTTGTTTCTAAATGAGGGATTTTTTGGTGTTTTGTTTTACATATATAAGTGTGTACCATATGTATCTAGTGCCCAATGATCAGAAGAGGGCGTTGGGTCTCTTGATAATTATGAGCCACCATGGGGGCATTGGAAACCAAATCCATGTTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGT(+ctt)GCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421183748_h4h2 ATTTTTGTTAGGATTTGTTTCTAAATGAGGGATTTTTTGGTGTTTTGTTTTACATATATAAGTGTGTACCATATGTATCTAGTGCCCAATGATCAGAAGAGGGCGTTGGGTCTCTTGATAATTATGAGCCACCATGGGGGCATTGGAAACCAAATCCATGTTCTCTGACAGAACAA(+ctt)GTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421183715_dx3b ATTTTTGTTAGGATTTGTTTCTAAATGAGGGATTTTTTGGTGTTTTGTTTTACATATATAAGTGTGTACCATATGTATCTAGTGCCCAATGATCAGAAGAGGGCGTTGGGTCTCTTGATAATTATGAGCCACCA(+aggat)TGGGGGCATTGGAAACCAAATCCATGTTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10