Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250926055625_m146 CCCGCACTCCCGGCGCCGCCACCGCACCACCTTCAGCCCAGTGCAGTTGGAACAGCTGGAGTCAGCCTC(/T)AGGGAGGAACCAGTACCCCGACATCTGGGCCCG NGG 10 8 17 8 24 0 100 5 10
    250925004634_kkas aaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/G)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttt NGG 10 8 17 8 24 0 100 5 10
    250925004600_7zfb aaacacatgaacagttcctctctaactttctgtttttggtaataggacatctccaagtttgcagagaaggacaatatcgtccttggagaaggtggaatcacactgagtggTgA(/G)Tcagcgagcaagaatttccttagcaaggtgaatatcccattattggttcagcgagcatttgttgtaaatatcatgcatgtaaaaattatagacatttctctgttttt NG 10 8 17 8 24 0 100 5 10
    250923175504_6xfg agcaaacctttacatagcagtggaatcaatgtaaatgcagctcC(/T)attccaatccatgcaaacggtaagcaaattaactaacatta NGG 10 8 17 8 24 0 100 5 10
    250923171902_w1k1 TCATTTTCTTCTGCTACTGAAATAACACCATCAGTGGCATTCACAGAAGTGCCATCTTTATTTCCTTCTAAAAAGAGTGCAAAAAGAACAATTTTATCCTCATCCTTGGAAGAATCCATTACCCTATC(-A)AAGTAATTTGGATGTTAATTTATGTTTGGATAAGACTTGTTTATCCATTGTCCCTTCACAAACTATCTCTTCAGACTTGATGAATTCTGATTTGACTTCAAAAATGACTACTGATGAACTGTCAGTATCAGA NG 10 8 17 8 24 0 100 5 10
    250923171851_txm6 TCATTTTCTTCTGCTACTGAAATAACACCATCAGTGGCATTCACAGAAGTGCCATCTTTATTTCCTTCTAAAAAGAGTGCAAAAAGAACAATTTTATCCTCATCCTTGGAAGAATCCATTACCCTATC(-A)AAGTAATTTGGATGTTAATTTATGTTTGGATAAGACTTGTTTATCCATTGTCCCTTCACAAACTATCTCTTCAGACTTGATGAATTCTGATTTGACTTCAAAAATGACTACTGATGAACTGTCAGTATCAGA NG 10 5 10 15 24 0 100 5 10
    250923171740_23qj TCATTTTCTTCTGCTACTGAAATAACACCATCAGTGGCATTCACAGAAGTGCCATCTTTATTTCCTTCTAAAAAGAGTGCAAAAAGAACAATTTTATCCTCATCCTTGGAAGAATCCATTACCCTATC(-A)AAGTAATTTGGATGTTAATTTATGTTTGGATAAGACTTGTTTATCCATTGTCCCTTCACAAACTATCTCTTCAGACTTGATGAATTCTGATTTGACTTCAAAAATGACTACTGATGAACTGTCAGTATCAGA NG 10 5 10 15 24 0 100 5 10
    250923101124_77au GTAGTAGGTCTTTGGCATTAGGAGCTTGAGCCCAGACGGCCCTAGCAGGGACCCCAGCGCCCGAGAGACC(+TG)ATGCAGAGGTCGCCTCTGGAAAAGGCCAGCGTTGTCTCCAAACTTTTTTTCAGGTGAGAAGGTGGCCAACCGAGCTTCGGAAAG NGG 10 8 17 8 24 0 100 5 10
    250922152428_3s8l ATGCTGTCGTCTTCCACCTCCACCCACTGCCTCCACCAGCCGCCGCCGCCGCCGGCGAGCCACCAATTCCGGTCGGCCGCCGCGTTCCGCCGCCctccgtctccggcggcggcgcccctccgcgcgcggccgcagcgcgggggcggcaccaccatccgcgccatcgacgcagcccagccgttcgactacgagtcccgcgcggcggggctcctggaggagcggcagcggctcaagatcgccatcgtcgggttcggcaacttcggccagttcctcgcccgcaccttcgcgcggcagggccacacgctgctcgcccactcccgctccgactactcctccctcgccgcatccgtcggcgcgtcctacttccaggacccgcacgacctctgcgagtgccacccggatgtggtgctcctcgCCACCTCCATCCTCTCCGCCGAGGCCGTCCTCCGCTCGCTCCCCGTCCACCGCCTCCGCCGCAACACCCTCTTCGTCGACGTGCTCTCCGTCAAGGAGTTCCCCAGGAACCTCCTCCTCGGCTCGCTCCCGCCGGACTTCGACGTCATCTGCACCCACCCCATGTTCGGCCCGGAG(/C)TCGGCGCGCGACGGCTGGGACGGCCTCCCCTTCGTGTTCGACAAGGTCCGCGTCGGCGACTGCCCCGCGCGCCGCGCCCGCGCCGAGGCCTTCCTCAACATCTTCGAGCGGGAGGGGTGCCGCATGGTGGAGATGACCTGCGCCGAGCACGACGCGCACGCCGCCGAGACGCAGTTCCTCACCCACACCGTCGGCCGGATGCTCGCCATGCTGGAGCTCCGGTCCACGCCGATCAACACCAAGGGGTACGAGACGCTGCTCCGCCTCGTCGACAACACCTGCAGCGACAGCTTCGACCTCTACAATGGCCTCTTCATGTACAACAAGAACTCCACCGAGCTGCTCAATCGCCTCGAATGGGCCATGGATTCCGTCAAGAAGAAGCTCTTCGATGGCCTCCACGACGTGCTCCGGAAGCAGCTCTTCGAGGGCTCGCCGCACGCTCCCAACAACACCCCCGTCAGGAAGTAG NGG 10 8 17 8 24 0 100 5 10
    250922135403_ltwn TTAGAAAGACTGCAAACTGGTACTTGAAAACTCTGCGTTTGTCCTGTCATTCCATTTGTAGTTAAGGGTTTCACCATATGACATCCTCGGTGTTAACCAGAGGCCTTGGTGCTGATTCACAAGAACCTAAG(+ct)CTGTCACTGAAATGAACTGAGAAAGTATCAGGTGGGGGGGGGGGGGAGAAGACAGCATGTTTCCATAGGAAAATGGACCAAAAGTGCAAGTTTTGGTTCAACATTATTGAAATCCTACAGGTCATTGCTGAACGAGTCAAAGAAAGGAAGGCAGAGGAAGACTGGACGGGTGCTGGCAGGGGTCCTATC NGG 10 8 17 8 24 0 100 5 10