Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250730172352_49rk ATAACCCCATGAGATGTGCAAAGTAAATGGGTTTAACTATTGCACAGTTGAAAAAACTGAAGCTTACAGAGGCTAAGGGCCTCCCCTGCTTGGCTGGGCGCAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCGGATCACGAGGTTGGGAGATCGAGACCATCCTGGCTAACGGTGAAACCCCGTCTCTACTGAAAAATACAAAAAAAAATTAGCCGGGCGTGGTGCTGGGCACCTGTAGTCCCAGCTACTCGGGAGGCTGAGGAAGGAGAATGGCGTGAACCTGGGCGGTGGAGCTTGCAGTGAGCTGAGATCACGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGATTCCATCTCAAAAAAAAAAAAAAAAGGCCTCCCCTGCTTGCCACAGGTCTCCCCAAGGCGCACTGGCCTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCCTGCATGGGCGGCATGAACCG(/A)GAGGCCCATCCTCACCATCATCACACTGGAAGACTCCAGGTCAGGAGCCACTTGCCACCCTGCACACTGGCCTGCTGTGCCCCAGCCTCTGCTTGCCTCTGACCCCTGGGCCCACCTCTTACCGATTTCTTCCATACTACTACCCATCCACCTCTCATCACATCCCCGGCGGGGAATCTCCTTACTGCTCCCACTCAGTTTTCTTTTCTCTGGCTTTGGGACCTCTTAACCTGTGGCTTCTCCTCCACCTACCTGGAGCTGGAGCTTAGGCTCCAGAAAGGACAAGGGTGGTTGGGAGTAGATGGAGCCTGGTTTTTTAAATGGGACAGGTAGGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACGGAACAGCTTTGAGGTGCGTGTTTGTGCCT NGG 10 8 17 8 24 0 100 5 10
    250730134309_tbhw GAGTACACACGCCTGGCCGACTACCTGTCGGCGCAGCGCGTGGACGGCGTGCTCCTGA(+A)TGTCGGTGCGCAGCGAGGACACCCTGCCCGACCGGCTGCGGGAGCTGGCGGTGCCGACCGTG NGG 10 8 17 8 24 0 100 5 10
    250730130913_wsks GAGTACACACGCCTGGCCGACTACCTGTCGGCGCAGCGCGTGGACGGCGTGCTCCTGA(+A)TGTCGGTGCGCAGCGAGGACACCCTGCCCGACCGGCTGCGGGAGCTGGCGGTGCCGACCGTG NGG 10 8 17 8 24 0 100 5 10
    250730130642_s79m GAGTACACACGCCTGGCCGACTACCTGTCGGCGCAGCGCGTGGACGGCGTGCTCCTGA(+A)TGTCGGTGCGCAGCGAGGACACCCTGCCCGACCGGCTGCGGGAGCTGGCGGTGCCGACCGTG NGG 10 8 17 8 24 0 100 5 10
    250730130301_ozam CCCAACCGCGCCGCGCGCTCCCTGGCGACCCGCCGCACCGACTCGGTGGCCCTGGTCGTGCCCGAGGCGGAGACCCGGCTGTTCACCGAGCCGTACTTCTCGGGCATCA(+A)TCCCGGGCGTGTCCGCCGGACTCGCGGACTCCGGGTTGCAGATGCTGCTGGTCCTGCCGCGGGACGAGAAG NGG 10 8 17 8 24 0 100 5 10
    250730125849_d720 CCCAACCGCGCCGCGCGCTCCCTGGCGACCCGCCGCACCGACTCGGTGGCCCTGGTCGTGCCCGAGGCGGAGACCCGGCTGTTCACCGAGCCGTAC(+A)TTCTCGGGCATCATCCCGGGCGTGTCCGCCGGACTCGCGGACTCCGGGTTGCAGATGCTGCTGGTCCTGCCGCGGGACGAGAAG NGG 10 8 17 8 24 0 100 5 10
    250730125644_f3cz AAGGTCAGCGCCCGCTCCCGGGCGGCGGTGGAACGGGCC(+A)ATCGCGGAACTCGGGTACGTCCCCAACCGCGCCGCGCGCTCCCTGGCGACCCGCCGCACCGACTCGGTGGCCCTGGTCGTG NGG 10 8 17 8 24 0 100 5 10
    250730125537_euul CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250728185716_65ft TGGGGGTGTATTACTTTGCCATGCCATTTGTTTCCTCCATGCGTAACTTAATCCAGACTTTCACACCTTATAGGAAAACCAGTGAGT(/C)CTGGAGAGCTGCATGGGCTCACAACTGAGGAGGAATTTGTAGAAGGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGGCACTTGGCATCTCCCCATTCCATGAGCATGCAGAG NGG 10 8 17 8 24 0 100 5 20
    250728185424_6347 TGGGGGTGTATTACTTTGCCATGCCATTTGTTTCCTCCATGCGTAACTTAATCCAGACTTTCACACCTTATAGGAAAACCAGTGAGT(/C)CTGGAGAGCTGCATGGGCTCACAACTGAGGAGGAATTTGTAGAAGGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGGCACTTGGCATCTCCCCATTCCATGAGCATGCAGAG NGG 10 8 17 8 24 0 100 5 10