Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260715085146_dl8s CGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGG(/C)GTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATG NGG 10 8 17 8 24 0 100 5 10
    260714165743_8hn9 CGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGG(/T)GTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATG NGG 10 8 17 8 24 0 100 5 10
    260714162208_094w CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    260714160929_logd GAACGACGGCTACAGGGGCTCCTCCAAGGACAAGTCGATGGCGGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGG(/T)GTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGC NGG 10 8 17 8 24 0 100 5 10
    260714160724_ul1u CGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGG(/C)GTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATG NGG 10 8 17 8 24 0 100 5 10
    260714155504_b50x GAACGACGGCTACAGGGGCTCCTCCAAGGACAAGTCGATGGCGGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCGG(/C)GTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGC NGG 10 8 17 8 24 0 100 5 10
    260714153708_jcoj GGAAGTTAAACTTAATTGTTTACATGGTGTGGCTGGTGTTTCCTCTTTTTTTTTCTCAATCTTCAGGTATTGTTCTTGACTCTGGTG(/A)ATGGTGTGAGCCACACTGTGCCCATTTACGAAGGATATGCCCTTCCTCATGCCATTCTTCGTTTGGATCTTGCTGGTCGGGACC NGG 10 8 17 8 24 0 100 5 10
    260714152802_cw8w TTACATGGTGTGGCTGGTGTTTCCTCTTTTTTTTTCTCAATCTTCAGGTATTGTTCTTGACTCTGGTG(/A)ATGGTGTGAGCCACACTGTGCCCATTTACGAAG NGG 10 8 17 8 24 0 100 5 10
    260714151546_1fc1 GAACGACGGCTACAGGGGCTCCTCCAAGGACAAGTCGATGGCGGCGGCGGCGGGGGCAGGGGAGCAGGAGGAGGAGGTGGACGAGCTGCTGGCGGCGCTCG(/A)GGTACAAGGTGCGTTCGTCGGATATGGCGGACGTCGCGCAGAAGCTAGAGCAGCTCGAGATGGCCATGGGGATGGGCGGCGCCTGCCCCACCGCTGATGACGGGTTCGTCTCGCACCTCGC NGG 10 8 17 8 24 0 100 5 10
    260714114230_pabb GGAAGTTAAACTTAATTGTTTACATGGTGTGGCTGGTGTTTCCTCTTTTTTTTTCTCAATCTTCAGGTATTGTTCTTGACTCTGGTG(/A)ATGGTGTGAGCCACACTGTGCCCATTTACGAAGGATATGCCCTTCCTCATGCCATTCTTCGTTTGGATCTTGCTGGTCGGGACC NGG 10 8 17 8 24 0 100 5 10