Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260926220640_6r7y GCTGGGATCAGGCAAGAGCCCCGCTCTCCTGGGCGCAGCTGCAGCCACCCAAGTCGTGGCCACAGAGCCAGGTGTCCCTGCACTCTCAGGGGCCCTGGTCCAAACCCAAGCCTCCAGGTGATCTTGCAAGCAGGGAGCACGCAGGAGACCCACCCTCCCGGGCACAGCTGCAGCTACCCAAGTTGTGGCTGTGGACCCAGGCATCCCTGCACTCTCAGGGTCTGGGAAGATCCCCCCTTGCCTCCGCAGGCT(/A)CAGAGCTGTCTGTTCCCACTGCTTGGCCTCTCCCCTGCTTCTGGCACCCGATCTGATCCTGGAGGCACAGTCGGGGCTACGCACTCTGTGGATCCAGTGTGAGTCGGGGACAAGCGGGACCCCTGCCCCTTCCGAGGTAGCCAGGAGCTCTCCTGGGGCAGCTGCAGCTGCCCAAGCCAGGACCCAGGCATCTCTACTGCCCCCACAAGCTCAGGGGTGTCTGCTCCCACTGTCTG NGG 10 8 17 8 24 0 100 5 10
    260926220623_al83 GCTGGGATCAGGCAAGAGCCCCGCTCTCCTGGGCGCAGCTGCAGCCACCCAAGTCGTGGCCACAGAGCCAGGTGTCCCTGCACTCTCAGGGGCCCTGGTCCAAACCCAAGCCTCCAGGTGATCTTGCAAGCAGGGAGCACGCAGGAGACCCACCCTCCCGGGCACAGCTGCAGCTACCCAAGTTGTGGCTGTGGACCCAGGCATCCCTGCACTCTCAGGGTCTGGGAAGATCCCCCCTTGCCTCCGCAGGCT(/A)CAGAGCTGTCTGTTCCCACTGCTTGGCCTCTCCCCTGCTTCTGGCACCCGATCTGATCCTGGAGGCACAGTCGGGGCTACGCACTCTGTGGATCCAGTGTGAGTCGGGGACAAGCGGGACCCCTGCCCCTTCCGAGGTAGCCAGGAGCTCTCCTGGGGCAGCTGCAGCTGCCCAAGCCAGGACCCAGGCATCTCTACTGCCCCCACAAGCTCAGGGGTGTCTGCTCCCACTGTCTG NGG 10 8 17 8 24 0 100 5 10
    260926215631_9ndr TCAACCGTTGAACCCTCATTGCAGCCACCAGAGTTCTGTGCCACCCCTGCTCCGTTCCCGCGGTGGAGGGAGGACGCCTAGGCCTTGGGAGGGATCGGGAGCGCGTAGAGTGACACCCTGGGCTCCACATCCACGACACCTTCTCCCCCATCTGGT(/A)CTGCCCAACTACTTCATGCCTACCTCGGGACTTTGCATGCAAAAGGCGCTCAATCATCCCTTCAAGAATAATTGAGTTAGAAAACAGCAGGGCACAATGACAGGTGGGCCCAGAAGTTGCCACCAGAGGGCAGTGCGCGCACCTCCCCAGCCTCCTCGAGACTTGTGCTGCAGGAGGGAGGTGAAGTCGCGGCCAGGAGCGTGACCACGCTGGACCGTGTCTGCTGCCTCCCCGGGAGTCGCAAGTGCCCACAGGACAAGCAGGGGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    260926213247_bygp CCTCTGCCCAGCTCCGCACCCCCGCCCCCTTAGAGATACGGCCTGGTCGGGTCCTGGTCCCCCCCTCGGCGGCGCTTCCACTTTTCCCAGACTGAGGGTGGGGAAAAGGAGGAGGGGGAAGAAATCAGAGGAGGAAAAGTCGGGAGGTAGGGGAGCCGGGAGACAGGAGAGGGGGAAAATAAAGAGCCTGAGACACAAACGAGAGGAAAAGACCATCACAGAAAGCTGG(/T)AAATCTCCGGAGAGGCCAGCGAGAACCCGCGCTCCCCACGGATTCCATCATTCCTTCCGAAGGCGCCTCTGCGGTGTCTCAGCCGTGCCAGGCCCCGGGGTTCCCAGGACGCGGAGGAGTGCTGGGTGCGGCCGCCTCGCCTCCCCACCCCTGGCCGCCCCTCCCCACCTCGCCCAAGGGGGCCGGAACGGCGTCGGCGCGCGGGGGCTTTTCGGAGCAGTCGAGTGGAAAATAGACTTTAACCCGCTTTGTGGCGGCCGGGGCGCCCTGAGCGCTCTCCAAACCACGG NGG 10 8 17 8 24 0 100 5 10
    260926211456_77xt AGCTGTCCCCCGTGACATCCAGAAAACGCGAAACCTCAGGAACAAGGTCGCAGCTTCAGACCGCGGCCCAGGAGGCCGATGGTGGGTGAGTGGGAGAGTCCCGGAGAGCAGGGGGGCAGAGAGCTGGTTTTCGGGAAAACCAATGTGTTGGACCCCAAACATCCACCCTCCGCTCGGATCCAAGTTCTCTGAGAACTGAAACGACATCCCGGGACGAATGGGAGAGTTAGGCTGAGCTACACACCGGGGAGGGGAGGGTTGGAG(/A)TTTAGCCCCAAGCCCTTCGGACGCCTTCTTCGGCTCCCGCGTGGGTTGAGACGGCGGCACGGCCACCAGACTCAGCTAAAGGGCGGAGTCGCGAGGAGAAGCCAGTGGCGAGGGGAGGAGGAGGCCTGGATCTCCCCGCGAAGGCTCCAGTCCGGCTTTTGCCTCCGACTGCGGGCTCCCTCCCCACCCGCCGTCCCTCGCCCCGCCCCGCC NGG 10 8 17 8 24 0 100 5 10
    260925184822_8v4j GGCCTCTGCAAGAAGCGCCCGAAGCCTGGAGGATGGAACACTGGGGGCAGCCGATACCCGGGGCAGGGCAGCCCTGGAGGCAACCGCTACCCACCTCAGGGCGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCCCATGGTGGTGGCTGGGGACAGCCTCATGGTGGTGGCTGGGGTCAAGGAGGTGGCACCCACAGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGG(/T)CTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCGGCAGTGACTATGAGGACCGTTACTATCGTGAAAACATGCACCGTTACCCCAACCAAGTGTACTACAGGCCCATGGATGAGTACAGCAACCAGAACAACTTTGTGCACGACTGCGTCAATATCACAATCAAGCAGCACACGGTCACCACAACCACCAAGGGGGAGAACTTCACCGAGACCGACGTTAAGATGATGGAGCGCGTGGTTGAGCAGATGTGTATCACCCAGTACGAGAGGGAATCTCAGGCCTATTA NGG 10 8 17 8 24 0 100 5 10
    260925184045_5b48 GGCCTCTGCAAGAAGCGCCCGAAGCCTGGAGGATGGAACACTGGGGGCAGCCGATACCCGGGGCAGGGCAGCCCTGGAGGCAACCGCTACCCACCTCAGGGCGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCCCATGGTGGTGGCTGGGGACAGCCTCATGGTGGTGGCTGGGGTCAAGGAGGTGGCACCCACAGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCG(/T)CTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCGGCAGTGACTATGAGGACCGTTACTATCGTGAAAACATGCACCGTTACCCCAACCAAGTGTACTACAGGCCCATGGATGAGTACAGCAACCAGAACAACTTTGTGCACGACTGCGTCAATATCACAATCAAGCAGCACACGGTCACCACAACCACCAAGGGGGAGAACTTCACCGAGACCGACGTTAAGATGATGGAGCGCGTGGTTGAGCAGATGTGTATCACCCAGTACGAGAGGGAATCTCAGGCCTATTA NGG 10 8 17 8 24 0 100 5 10
    260920160029_kiye ATGGATTGGGGCACGCTGCAGACGATCCTGGGGGGTGTGAACAAACACTCCACCAGCATTGGAAAGATCTGGCTCACCGTCCTCTTCATTTTTCGCATTATGATCCTCGTTGTGGCTGCAAAGGAGGTGTGGGGAGATGAGCAGGCCGACTTTGTCTGCAACACCCTGCAGCCAGGCTGCAAGAACGTGTGCTACGATCACTACTTCCCCATCTCCCACATCCGGCTATGGGCC(-C)TGCAGCTGATCTTCGTGTCCACGCCAGCGCTCCTAGTGGCCATGCACGTGGCCTACCGGAGACATGAGAAGAAGAGGAAGTTCATCAAGGGGGAGATAAAGAGTGAATTTAAGGACATCGAGGAGATCAAAACCCAGAAGGTCCGCATCGAAGGCTCCCTGTGGTGGACCTACACAAGCAGCATCTTCTTCCGGGTCATCTTCGAAGCCGCCTTCATGTACGTCTTCTATGTCATGTACGACGGCTTCTCCATGCAGCGGCTGGTGAAGTGCAACGCCTGGCCTTGTCCCAACACTGTGGACTGCTTTGTGTCCCGGCCCACGGAGAAGACTGTCTTCACAGTGTTCATGATTGCAGTGTCTGGAATTTGCATCCTGCTGAATGTCACTGAATTGTGTTATTTGCTAATTAGATATTGTTCTGGGAAGTCAAAAAAGCCAGTTTAA NGG 10 8 17 8 24 0 100 5 10
    260914210122_tmc4zx7l TATTTAATAAGTCATCGGTGATTGGTCCGCCCCTGAGGTTAATCTTAAAAGCCCAGGTTACCCGCGGAAATTTATGCTGTCCGGTCACCGTGACAATGCAGCTGAGGAACCCAGAACTACATCTGGGCTGCGCGCTTGCGCTTCGCTTCCTGG(/C)CCCTCGTTTCCTGGGACATCCCTGGGGCTAGAGCACTGGACAATGGATTGGCAAGGACGCCTACCATGGGCTGGCTGCACTGGGAGCGCTTCATGTGCAACCTTGACTGCCAGGAAGAGCCAGATTCCTGCATCAGGTATCAGATAT NGG 10 8 17 8 24 0 100 5 10
    260914210122_tmc4x2nt ATGTTTCCTGATGGTCCATGTCTGTTACTCGCCTGTCAAGTGGCGTGACACCGGGCGTGTTCCCCAGAGTGACTTTTCCTTTTATTTCCCTTCAGCTAAAATAAAGGAGGAGGAAGCTGCTAAGGACTAGTTCTGCCCTCCCGTCACCCCTGT(+GAT)TTCTGGCACCAGGAATCCCCAACATGCACTGATGTTGTGTTTTTAACATGTCAATCTGTCCGTTCACATGTGTGGTACATGGTGTTTGTGGCCTTGGCTGACATGAAGCTGTTGTGTGAGGTTCGCTTATCAACTAATGATTTAGTG NGG 10 8 17 8 24 0 100 5 10