Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    251114161945_pf9t CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    251112193911_qg11 CTCGACTTGTCCCTCTGGGCACCCATGAGGACCCCTCCCTTATGATCAGGCTTCCGGGTTCCTGTGCCCTGACAAGCCCCTGTGACCAGCCCTGCAGGTGACTTGTTTGTGTCCCCAGATTACACTGACGACCATCGG(/A)CTATGGTGACAAGACGCCACACACCTGGCTGGGCAGGGTCCTGGCTGCTGGCTTTGCCTTACTGGGCATCTCCTTCTTTGCCTTGCCTGCTGTGAGTGCCCT NGG 10 8 17 8 24 0 100 5 10
    251112060103_ux6e CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251107124646_bv1r CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NG 10 8 17 8 24 0 100 5 10
    251107014658_p8bo CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251107002935_m078 ctactggaacaggtggtggcgggcctcggcgcgctcgtactgctccaccacggtgtagtcctcgttgtgggaggtgatgtccagcttggagtccacgtagtagtagccgggcagcttcacgggcttcttggccatgtagattgacttgaactccaccaggtagtggccgccgcccttcagcttcagcgccttgtggatctcgcccttcagcacgccgtcgcgggggtacaggcgctcggtggagggctcccagcccagagtcttcttctgcattacggggccgtcggaggggaagttcacgccgatgaacttcacgtggtagatgaaggtgccgtcttgcagggaggagtcctgggtcacggtcaccacgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggacagcttcttgtagtcggggatgtcggcggggtgcttcacgtacaccttggagccgtactggaactggggggacaggatgtcccaggcgaagggcagggggccgcccttggtcacttgcagcttggcggtctgggtgccctcC(/G)tagggcttgccctcgccctcgccctcgatctcgaactcgtggccgttcacggagccctccatgtgcaccttgaagcgcatgaagggcttgatgacgttctcagtgctatccat NGG 10 8 17 8 24 0 100 5 10
    251107000931_uvvr atggatagcactgagaacgtcatcaagcccttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggcaagccctaG(/C)gagggcacccagaccgccaagctgcaagtgaccaagggcggccccctgcccttcgcctgggacatcctgtccccccagttccagtacggctccaaggtgtacgtgaagcaccccgccgacatccccgactacaagaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaagacggcaccttcatctaccacgtgaagttcatcggcgtgaacttcccctccgacggccccgtaatgcagaagaagactctgggctgggagccctccaccgagcgcctgtacccccgcgacggcgtgctgaagggcgagatccacaaggcgctgaagctgaagggcggcggccactacctggtggagttcaagtcaatctacatggccaagaagcccgtgaagctgcccggctactactacgtggactccaagctggacatcacctcccacaacgaggactacaccgtggtggagcagtacgagcgcgccgaggcccgccaccacctgttccagtag NGG 10 8 17 8 24 0 100 5 10
    251106220133_55uc TTCTTTATTTTAGTTTGGCATTTGTTGACTGATAATTAATCGCCCTGTATCaatgtttgtttccttttttcttctctCCAGAGGCCACAATCAGAGAATGG(/A)AATTCCTGGGTGATTCCATAATGCAGCTGGTAGCCACAgaatatttattcatacatttccCTGATCATCATGAAGGCCACTTAACGGTGAGAGCCACATA NGG 10 8 17 8 24 0 100 5 10
    251106215936_zfqd TTTGGCATTTGTTGACTGATAATTAATCGCCCTGTATCaatgtttgtttccttttttcttctctCCAGAGGCCACAATCAGAGAATGGAATTCCTGGGTGA(/G)TTCCATAATGCAGCTGGTAGCCACAgaatatttattcatacatttccCTGATCATCATGAAGGCCACTTAACGGTGAGAGCCACATAAATCACGCTCTGA NGG 10 8 17 8 24 0 100 5 10
    251106214329_o1kd TTCTTTATTTTAGTTTGGCATTTGTTGACTGATAATTAATCGCCCTGTATCaatgtttgtttccttttttcttctctCCAGAGGCCACAATCAGAGAATGG(/A)AATTCCTGGGTGATTCCATAATGCAGCTGGTAGCCACAgaatatttattcatacatttccCTGATCATCATGAAGGCCACTTAACGGTGAGAGCCACATA NGG 10 8 17 8 24 0 100 5 10