Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260601142023_x6oy CTCCATTTAAGCAAAATCCTTCTTTGGCAAAGTCTAGTTTTAAAAGTAAACTGCTTGCCAAACAACAATTTGTTAGGAATTCTTGGGGGAATTGGTTTCTAATATTGGTGTGGGGCTGAACCCTCAGCCAATACGTTGATCCATTATTATCTGAAGGGGGGGGGGGCTTGAAATCCAAGAATATTGGAATCTGTTTCTTAAAGTGACATATTAGTTAGAAAACATCTGTGAACATTCTTCAGTTTACTTCGGATCGCCTCGAGATTCACAGTACCTACTGCAATGCATTAATAAAAGCAGCGAGTGTATCGTGTTGGATGGTTTAGGGAATAGTCACAAGGAAAACTGTCTGTATACATTCAGTGCAGATACGGGTTTTGTTGTTGTTGTTGTTGTTGTTGTTGTCGTTGTCGTTGTCGTCGTCGTCGTTGTTGTTCATTTGTTTTTTTTGCCAAACGTTTTTTGATCTGCAGCTAGTTGAATCCACAGTGTCAAACTGCCTGTATCGCAATACATTCATTCTCTATAGTATAGCAAGTTACATCTACTTCAATTTCTAACCATCAATATAAAGGATAGACTTGAATAAAACTGTAGAGTCATATTTGTATGCACGATCCATGATAACTATTGTGATTCTGTGACCTGGATGTTTGTTTGAACTGTAGATTTTTTTTTTCTGAAGCAGATACGTTCCATTTGTTTAGAGCACAAATTGGAAA(+atggtc)GGGTGGTGATGTTTTCGTTTCTGGGGTAAAAACTTGGCAGATGCCTTGGGGGCTTGGTGCTGACCTCTGTCTGGTTGAAACCAGATCTACCTGATGCTGCACATTCACTGGAAATTTAAAATCTTAATTGGCTTCTATCAGTGAAAGGTGAATGAAATTAATAACAACTTCTCCTTCAATGCAG NGG 10 8 17 8 24 0 100 5 10
    260601141951_qxzo CTCCATTTAAGCAAAATCCTTCTTTGGCAAAGTCTAGTTTTAAAAGTAAACTGCTTGCCAAACAACAATTTGTTAGGAATTCTTGGGGGAATTGGTTTCTAATATTGGTGTGGGGCTGAACCCTCAG(+cttatg)CCAATACGTTGATCCATTATTATCTGAAGGGGGGGGGGGCTTGAAATCCAAGAATATTGGAATCTGTTTCTTAAAGTGACATATTAGTTAGAAAACATCTGTGAACATTCTTCAGTTTACTTCGGATCGCCTCGAGATTCACAGTACCTACTGCAATGCATTAATAAAAGCAGCGAGTGTATCGTGTTGGATGGTTTAGGGAATAGTCACAAGGAAAACTGTCTGTATACATTCAGTGCAGATACGGGTTTTGTTGTTGTTGTTGTTGTTGTTGTTGTCGTTGTCGTTGTCGTCGTCGTCGTTGTTGTTCATTTGTTTTTTTTGCCAAACGTTTTTTGATCTGCAGCTAGTTGAATCCACAGTGTCAAACTGCCTGTATCGCAATACATTCATTCTCTATAGTATAGCAAGTTACATCTACTTCAATTTCTAACCATCAATATAAAGGATAGACTTGAATAAAACTGTAGAGTCATATTTGTATGCACGATCCATGATAACTATTGTGATTCTGTGACCTGGATGTTTGTTTGAACTGTAGATTTTTTTTTTCTGAAGCAGATACGTTCCATTTGTTTAGAGCACAAATTGGAAAGGGTGGTGATGTTTTCGTTTCTGGGGTAAAAACTTGGCAGATGCCTTGGGGGCTTGGTGCTGACCTCTGTCTGGTTGAAACCAGATCTACCTGATGCTGCACATTCACTGGAAATTTAAAATCTTAATTGGCTTCTATCAGTGAAAGGTGAATGAAATTAATAACAACTTCTCCTTCAATGCAG NGG 10 8 17 8 24 0 100 5 10
    260601141915_6uhp CTCCATTTAAGCAAAATCCTTCTTTGGCAAAGTCTAGTTTTAAAAGTAAACTGCTTGCCAAACAACAATTTGTTAGGAATTCT(+cttatg)TGGGGGAATTGGTTTCTAATATTGGTGTGGGGCTGAACCCTCAGCCAATACGTTGATCCATTATTATCTGAAGGGGGGGGGGGCTTGAAATCCAAGAATATTGGAATCTGTTTCTTAAAGTGACATATTAGTTAGAAAACATCTGTGAACATTCTTCAGTTTACTTCGGATCGCCTCGAGATTCACAGTACCTACTGCAATGCATTAATAAAAGCAGCGAGTGTATCGTGTTGGATGGTTTAGGGAATAGTCACAAGGAAAACTGTCTGTATACATTCAGTGCAGATACGGGTTTTGTTGTTGTTGTTGTTGTTGTTGTTGTCGTTGTCGTTGTCGTCGTCGTCGTTGTTGTTCATTTGTTTTTTTTGCCAAACGTTTTTTGATCTGCAGCTAGTTGAATCCACAGTGTCAAACTGCCTGTATCGCAATACATTCATTCTCTATAGTATAGCAAGTTACATCTACTTCAATTTCTAACCATCAATATAAAGGATAGACTTGAATAAAACTGTAGAGTCATATTTGTATGCACGATCCATGATAACTATTGTGATTCTGTGACCTGGATGTTTGTTTGAACTGTAGATTTTTTTTTTCTGAAGCAGATACGTTCCATTTGTTTAGAGCACAAATTGGAAAGGGTGGTGATGTTTTCGTTTCTGGGGTAAAAACTTGGCAGATGCCTTGGGGGCTTGGTGCTGACCTCTGTCTGGTTGAAACCAGATCTACCTGATGCTGCACATTCACTGGAAATTTAAAATCTTAATTGGCTTCTATCAGTGAAAGGTGAATGAAATTAATAACAACTTCTCCTTCAATGCAG NGG 10 8 17 8 24 0 100 5 10
    260601130339_2w6w GTAAGGACTTCCCTTAATGTCTGTCCGCTCACCTGAAGTGTTATAAGTAGGCTAAACAGGATAAACACAAAGTATATATTTCTGATTAATATTCATAGGATTCTTACCTGCT(+agg)ATATCTCTCTGTTTTGTTTTTCTCCTTCATCATATTTTAG NGG 10 8 17 8 24 0 100 5 10
    260601130314_meb2 GTAAGGACTTCCCTTAATGTCTGTCCGCTCACCTGAAGTGTTATAAGTAGGCTAAACAGGATAAACACAAAGTATATATTTCTGATTAATATT(+ctt)CATAGGATTCTTACCTGCTATATCTCTCTGTTTTGTTTTTCTCCTTCATCATATTTTAG NGG 10 8 17 8 24 0 100 5 10
    260601130243_aizr GTAAGGACTTCCCTTAATGTCTGTCCGCTCACCTGAAGTGTTATAAGTAGGCTA(+ctt)AACAGGATAAACACAAAGTATATATTTCTGATTAATATTCATAGGATTCTTACCTGCTATATCTCTCTGTTTTGTTTTTCTCCTTCATCATATTTTAG NGG 10 8 17 8 24 0 100 5 10
    260601130217_bb4j GTAAGGACTTCCCTTAATGTCTGTCCGCTCACCTGAAGTGTTATA(+cttatg)AGTAGGCTAAACAGGATAAACACAAAGTATATATTTCTGATTAATATTCATAGGATTCTTACCTGCTATATCTCTCTGTTTTGTTTTTCTCCTTCATCATATTTTAG NGG 10 8 17 8 40 0 100 5 10
    260601124654_au4b GTAAGGCCGGGGTGTGTGGTAGCTCCGACCCCGGCGGCTCCCGGTGTCCCAGAGTGCACAG(+cttatg)TCCTGGCCTCACCGCCACCGGCACGGGCAGTCTGCCAAATCAGACTGAGCATCGCTGGGCGCCTGAGCCTGGCCAGGGCGCACG NGG 10 8 17 8 40 0 100 5 10
    260601124557_ue87 GTAAGGCCGGGGTGTGTGGTAGCTCCGACCCCGGCGGCTCCCGGTGTCCCAGAGTGCACAG(+cttatg)TCCTGGCCTCACCGCCACCGGCACGGGCAGTCTGCCAAATCAGACTGAGCATCGCTGGGCGCCTGAGCCTGGCCAGGGCGCACG NGG 10 8 17 8 24 0 100 5 10
    260531232800_0f3q TCTTTTGTTTTATTTCAGACGATAATTGCTACTGCCATTTCATATGGAGCCAACCTGGAAAAAAATTACAATGCTGGCATTGTTAAATCCATCCCAAGGGG(/T)GTGAGTGTGGTGTTCCTCTTAGTACTAATACATTAAGTCAGTAAGTCAGTCTTTTTTATTTAAATAAAACCTTTTATTACAAGCTTCATTTCACTGATAC NGG 10 8 17 8 24 0 100 5 10