Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250422190400_lc5h CCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAGTCTGTGACTTGCACG(/T)GTCAGTTGCCCTGAGGGGCTGGCTTCCATGAGACTTCAATGCCTGGCCGTATCCCCCTGCATTTCTTTTGTTTGGAACTTTGGGATTCCTCTTCACCCTT NGG 10 8 17 8 24 0 100 5 10
    250422190207_ph7o CCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAGTCTGTGACTTGCACG(/C)GTCAGTTGCCCTGAGGGGCTGGCTTCCATGAGACTTCAATGCCTGGCCGTATCCCCCTGCATTTCTTTTGTTTGGAACTTTGGGATTCCTCTTCACCCTT NGG 10 8 17 8 24 0 100 5 10
    250422092052_2348 AAGTTGGATATTTTGTTATATAACTATAACCAATACTAACTCCACTGGGTGGATTTTTAATTCAGTCAGTAGTCTTAAGTGGTCTTTATTGGCCCTTCATTAAAATCTACTGTTCACTCTAA(+ctt)CAGAGGCTGTTGGTACTAGTGGCACTTAAGCAACTTCCTACGGATATACTAGCAGATTAAGGGTCAGGGATAGAAACTAGTCTAGCGTTTTGTATACCTACCAGCTTTATACTACCTTGTTCTGATAGAAATATTTCAGGACATCTAGAGTGTACTATAAGGTTGATGGTAAGCTTATAAGGAACTTGAAAGTGGAGT NGG 10 8 17 8 24 0 100 5 10
    250422052916_p35n CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250421192946_lqpp CACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGGCAGAGGACCCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCTAGC(+aag)CTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGG NGG 10 8 17 8 24 0 100 5 10
    250421192850_only CACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGG(+ctt)CAGAGGACCCAAGTTCAATTTCCAGCATACACCAGGCAGTAGTCACAACTACCCATAAGCTCCACCTAGCCTCCAACACAGCCTGCATTCAGTACACACACACATATATAAGTTAAAGGTCTTTTAAAAACAAAAACGGGAGTTATGGTGCTGGAGAGATAGCTCAGGGTAAGAGCAGTGTATTGCTCTTGAGGGGACCTGGGTCCAGGCACACACTTGGCACACATACATACATGAAGACAAGACACTCAGAAGTAAATCTATAAAGGGGGTCACCTATTTTTATTTTCTGTGGGTATTCATGTCTCAGTGCATTGTGGTGCCAGGC NGG 10 8 17 8 24 0 100 5 10
    250421192800_slm2 TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAGTGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCT(+aag)CTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAGCACTCCAGAGGCAGAGGAAGGCAGAGTTGTACCTGGCCTACATAGTAACTTCCAGGACAGCCAGGGCTACATAGTGAAATCTTGTCTCAAAAAACAAAGTTTAATTAAGCTAGAGACATGAATTCTTTGTTAAGAGTGCTCAGTACACTTGGCAGAGGAC NGG 10 8 17 8 24 0 100 5 10
    250421192609_spc0 TTCTCTGACAGAACAAGTGGGGTTGGTTGGTTTTTAGTTTAAGACTAGGGTTTCTCAGTATAGCCTTGGCTACCCTCAGACTCCCCAG(+ctt)TGCAGGGATTAAAGCACCACTACCACCAGCCCAGAACACATGTTGGCCTAATTGCTGAACCATCTCTCCAGCTTCCCAGTCCCCATTTTGATATTTTTATTCTACACATAGGAGAATACCTGCCTCTATCTCTGGAATGCTGGGATTATAGACACATGCCACCAAACCTAGCCTTATAACTTAATTTTTTAGAAAGATTTTTGCCAGTAGGGGTAGCACACAAATTTACCAG NGG 10 8 17 8 24 0 100 5 10
    250421192512_n6r7 GCTTTTTCTACCTGAAACTTAGTCTCAAAAAAAAATGAATACGTAAACAGTCTTCCAGGGGATAAGAACCTTACAGAAAAAGCAGAAATGCCTGGGGCACTGGATTACCGATGTAATCAAATTCAGTCCTTGAATTGAACACAGGATTGCCTAGAGCAAGGCCAGCCAGAGATTCATCTCAGAGGGAGAAAGGTGTCTTTGGAGCAATTTTGTGGTAATCTAGTATGTATCACATAAGTTTAGACGCATTTGGGACTGGAAAGATGTGAACAAAGCACCCTATGGCTCACATCTGTCATTAACTCTAGTTCCAGTGCACCTGACACCGTCT(+aag)TCTGGCCTCTGCAGTGACCAAGCACATGGGTAGTATGTAGACATATACATAAGCAAAACACACATCATTAAAAAGTGACATTTCCCAAAGGAAGCTGAAGAACCAGTTCTTGAGAAGATAGTAGAAATCAGAAGGGGAAATAGTAGACA NGG 10 8 17 8 24 0 100 5 10
    250421192347_tqk7 GCTTTTTCTACCTGAAACTTAGTCTCAAAAAAAAATGAATACGTAAACAGTCTTCCAGGGGATAAGAACCTTACAGAAAAAGCAGAAATGCCTGGGGCACTGGATTACCGATGTAATCAAATTCAGTCCTTGAATTGAACACAGGATTGCCTAGAGCAAGGCCAGCCAGAGATTCATCTCAGAGGGAGAAAGGTGTCTTTGGAGCAATTTTGTGGTAATCTAGTATGTATCACATAAGTTTAGACGCATTTGGGACTGGAAAGATGTGAACAAAGCACC(+ctt)gTATGGCTCACATCTGTCATTAACTCTAGTTCCAGTGCACCTGACACCGTCTTCTGGCCTCTGCAGTGACCAAGCACATGGGTAGTATGTAGACATATACATAAGCAAAACACACATCATTAAAAAGTGACATTTCCCAAAGGAAGCTGAAGAACCAGTTCTTGAGAAGATAGTAGAAATCAGAAGGGGAAATAGTAGACA NGG 10 8 17 8 24 0 100 5 10