Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250808042110_j72i CCACTAGCTTAGTTCTGTAAATGCTGAGTCATTTCCAGATTGGAGAATGATTTAAGCAAAGATGGTGAGGAGGTTAGAAAGCTCTTAGGAAACGGTTGTCAACGGTAAACTCAT(/C)GTCCCAGTTTGAAACATGAAACACTGTGAAACTATGAGAGGGCAGAAAAATAAGACTTCGGAAAAATGGAGGTGAGGGTCTAGAAGCCTCAATGAAGTAGTAAAACAGGGTCTGGAAGGCGGGGCTGGTAGCAAAAGAAAAGAAGGTGTGTGATTCCAGATGCGGATTCCCAAGGTCAAAGTTTCAGTTGCCACTCAAGCAATCTAACCCTTCTAACTGTCATCAT NGG 10 8 17 8 24 0 100 5 10
    250808040524_erfd GAACTCAAATATTTTTTTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(+GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGATATATCATGAAAGTTTC NG 10 8 17 8 74 0 100 7 10
    250808040458_jazh GAACTCAAATATTTTTTTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(+GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGATATATCATGAAAGTTTC NG 10 8 17 8 74 0 100 10 10
    250808040419_qn1s GAACTCAAATATTTTTTTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(+GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGATATATCATGAAAGTTTC NG 10 8 17 8 74 0 100 5 10
    250808035442_57mm GAACTCAAATATTTTTTTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(-GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGATATATCATGAAAGTTTC NGG 10 8 17 8 24 0 100 5 10
    250808035215_juoy GAACTCAAATATTTTTTTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(-GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGATATATCATGAAAGTTTC NG 10 8 17 10 74 0 100 7 10
    250808035125_29io GAACTCAAATATTTTTTTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(-GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGATATATCATGAAAGTTTC NG 10 8 17 8 74 0 100 5 10
    250808035051_mv4o GAACTCAAATATTTTTTTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(-GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGATATATCATGAAAGTTTC NG 10 8 17 8 40 0 100 5 10
    250808021746_pz3o GAACTCAAATATTTTTTTTTTAACAGATTTGGTTCATTTGACTTGCACATCTTCTAAAACAGCAAGAGAAGATTTAGAATCAGTTGTGCAGAAATTGTTT(-GTTC)CATATACTGAAATGGAGATAGGTAAGAAAAATAATTGATAACTCATGATGGAAAGGTAATGTAACCATCATAATCTTCTTAGATATATCATGAAAGTTTC NG 10 8 17 10 74 0 100 7 10
    250806194832_x92b GAACAGCTGGAGTCAGCCTTTGGGAGGAACCAGTACCCCGACATCTGGGCCCGAGAGAGT(+CT)TGCCCGGGACACTGGCCTCAGTGAGGCCCGAATCCAGgtgatgctccaggatcctcctct NGG 10 5 8 8 40 0 100 5 10