Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    251204143825_2cy9 CACAACAGCTTCATGTCAGCCAAGGCCACAAACACCATGTACCACACATGTGAACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGCCAGAA(+GATTACAAGGATGACGACGATAAG)ACAGGGGTGACGGGAGGGCAGAACTAGTCCTTAGCAGCTTCCTCCTCCTTTATTTTAGCTGAAGGGAAATAAAAGGAAAAGTCACTCTGGGGAACACGCCCGGTGTCACGCCACTTGACAGGCGAGTAACAGACATGGACCATCAGGAAACATTAACGT NGG 10 8 17 8 46 0 100 5 10
    251204143729_dhcp TGCTTGAATATAAATTTATTATTACTCTATGTTCTATTTAAGTTTTCATGTTCTAAAAATGTATCCCAGTTTACACGTCTCATATGCCCCTTGGCAGTCATCTTAGTCATTAC(+GATTACAAGGATGACGACGATAAG)CTGAGGTGTTCGTTGTAACTCATATAAACTGAGTTCCCATGTTTTGCTTAATGGTTGAGTTCCGTTTGTCTGCACAGCCTGAGACATTGCTGGAAATAAAGAAGAGAGAAAAACAATTTTAGTATTTGGAAGGGAAGTGCTATGGTCTGAATGTATGTGTCCCACCAAAATTCCTACGT NGG 10 8 17 10 46 0 100 5 10
    251204143630_jb26 CAGGGAAACGCCCATGCAATTAGTCTATTTCTGCTGCAAGTAAGCATGCATTTGTAGGCTTGATGCTTTTTTTCTGCTTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACG(+GATTACAAGGATGACGACGATAAGTGA)TGGCAGAGGAAAGGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAGCTTTTCCTAGACAGGGGCTAGTATGTGCAGCTCCTGCACCGGGATACTGGTTGACAAGTTTGGCTGGGCTGGAAGCCAGCACCTAGGGAGGTCCCTGGAAGGGGCCAGCCTCACCAGGAGAGGAGGGACCTGGCCCTTCAGGGTCGAGCTCAACAGAG NGG 10 8 17 10 47 0 100 5 10
    251204143435_xeq8 CACAACAGCTTCATGTCAGCCAAGGCCACAAACACCATGTACCACACATGTGAACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGCCAGAA(-ACAGGGGTGACGGGA)GGGCAGAACTAGTCCTTAGCAGCTTCCTCCTCCTTTATTTTAGCTGAAGGGAAATAAAAGGAAAAGTCACTCTGGGGAACACGCCCGGTGTCACGCCACTTGACAGGCGAGTAACAGACATGGACCATCAGGAAACATTAACGT NGG 10 8 17 8 24 0 100 5 10
    251204143337_20c8 CACAACAGCTTCATGTCAGCCAAGGCCACAAACACCATGTACCACACATGTGAACGGACAGATTGACATGTTAAAAACACAACATCAGTGCATGTTGGGGATTCCTGGTGCCAGAAACAGG(/T)GGTGACGGGAGGGCAGAACTAGTCCTTAGCAGCTTCCTCCTCCTTTATTTTAGCTGAAGGGAAATAAAAGGAAAAGTCACTCTGGGGAACACGCCCGGTGTCACGCCACTTGACAGGCGAGTAACAGACATGGACCATCAGGAAACATTAACGT NGG 10 8 17 8 24 0 100 5 10
    251204143208_4cdv ACGTCTCATATGCCCCTTGGCAGTCATCTTAGTCATTAC(-CTGAGGTGTTCGTTG)TAACTCATATAAACTGAGTTCCCATGTTTTGCTTAATGGTTGAGTTCCGTTTGTCTGCACAGCCTGAGACATTGCTGGAAATAAAGAAGAGAGAAAAACAATTTTAGTATTTGGAAGGGAAGTGCTATGGTCTGAATGTATGTGTCCCACCAAAATTCCTACGT NGG 10 8 17 8 24 0 100 5 10
    251204143039_mwo5 TGCTTGAATATAAATTTATTATTACTCTATGTTCTATTTAAGTTTTCATGTTCTAAAAATGTATCCCAGTTTACACGTCTCATATGCCCCTTGGCAGTCATCTTAGTCATTACC(/A)TGAGGTGTTCGTTGTAACTCATATAAACTGAGTTCCCATGTTTTGCTTAATGGTTGAGTTCCGTTTGTCTGCACAGCCTGAGACATTGCTGGAAATAAAGAAGAGAGAAAAACAATTTTAGTATTTGGAAGGGAAGTGCTATGGTCTGAATGTATGTGTCCCACCAAAATTCCTACGT NGG 10 8 17 8 24 0 100 5 10
    251204142753_6ttp CAGGGAAACGCCCATGCAATTAGTCTATTTCTGCTGCAAGTAAGCATGCATTTGTAGGCTTGATGCTTTTTTTCTGCTTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACG(-TGATGGCAGAGGAAA)GGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAGCTTTTCCTAGACAGGGGCTAGTATGTGCAGCTCCTGCACCGGGATACTGGTTGACAAGTTTGGCTGGGCTGGAAGCCAGCACCTAGGGAGGTCCCTGGAAGGGGCCAGCCTCACCAGGAGAGGAGGGACCTGGCCCTTCAGGGTCGAGCTCAACAGAG NGG 10 8 17 8 24 0 100 5 10
    251204141810_uggj CAGGGAAACGCCCATGCAATTAGTCTATTTCTGCTGCAAGTAAGCATGCATTTGTAGGCTTGATGCTTTTTTTCTGCTTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACGT(/A)GATGGCAGAGGAAAGGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAGCTTTTCCTAGACAGGGGCTAGTATGTGCAGCTCCTGCACCGGGATACTGGTTGACAAGTTTGGCTGGGCTGGAAGCCAGCACCTAGGGAGGTCCCTGGAAGGGGCCAGCCTCACCAGGAGAGGAGGGACCTGGCCCTTCAGGGTCGAGCTCAACAGAG NGG 10 8 17 8 24 0 100 5 10
    251202214609_lmms CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10