Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250928022420_a5m9 AGTTGGAACAGCTGGAGTCAGCCTTTGGGAGGAACCAGTACCCCGACATCTGGGCCCGAGAG(+AG)TCTTGCCCGGGACACTGGCCTCAGTGAGGCCCGAATCCA NGG 15 6 9 8 74 0 100 5 20
    250928021115_kgzq CCGCACTCCCGGCGCCGCCACCGCACCACCTTCAGCCCAGTGCAGTTGGAACAGCTGGAGTCAGCCTC(/T)AGGGAGGAACCAGTACCCCGACATCTGGGCCCG NGG 10 6 9 8 74 0 100 5 20
    250928020031_tuo6 CCGCACTCCCGGCGCCGCCACCGCACCACCTTCAGCCCAGTGCAGTTGGAACAGCTGGAGTCAGCCTC(/T)TGGGAGGAACCAGTACCCCGACATCTGGGCCCG NGG 10 8 17 8 24 0 100 5 20
    250928015837_9o6g CCGCACTCCCGGCGCCGCCACCGCACCACCTTCAGCCCAGTGCAGTTGGAACAGCTGGAGTCAGCCTC(/T)AGGGAGGAACCAGTACCCCGACATCTGGGCCCG NGG 10 8 17 8 24 0 100 5 20
    250928014135_1y1c CCGCACTCCCGGCGCCGCCACCGCACCACCTTCAGCCCAGTGCAGTTGGAACAGCTGGAGTCAGCCTC(/T)TGGGAGGAACCAGTACCCCGACATCTGGGCCCG NGG 10 6 9 8 74 0 100 5 20
    250928000951_abp3 AGTTGGAACAGCTGGAGTCAGCCTTTGGGAGGAACCAGTACCCCGACATCTGGGCCCGAGAG(+AG)TCTTGCCCGGGACACTGGCCTCAGTGAGGCCCGAATCCA NGG 15 8 17 8 70 0 100 5 20
    250927195926_himr CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+AT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250927195219_nr2h CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250927050137_ok8v gaggaggatcctggagcatcacCTGGATTCGGGCCTCACTGAGGCCAGTGTCCCGGGCAAGA(+CT)CTCTCGGGCCCAGATGTCGGGGTACTGGTTCCTCCCAAAGGCTGACTCCAGCTGTTCCAACTGCACTGGGCTGAAGGTGGTGCGGTGGCGGCGCCGGGAG NGG 15 8 17 8 70 0 100 5 10
    250927050049_iwqk gaggaggatcctggagcatcacCTGGATTCGGGCCTCACTGAGGCCAGTGTCCCGGGCAAGA(+CT)CTCTCGGGCCCAGATGTCGGGGTACTGGTTCCTCCCAAAGGCTGACTCCAGCTGTTCCAACTGCACTGGGCTGAAGGTGGTGCGGTGGCGGCGCCGGGAG NGG 15 8 17 8 70 0 100 5 10