Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    251201160950_kfzg ATGTGTTTGTTTCCAGCCTTGGGCATAGTCAGGGACTCTCGTGGGACCCCCCGAGTAGAAACACAGATGTGTCTCCCTGGGTCCCTGCCAGGTCCCCTCTC(+AGCCTGGATGGCTTCCCTCCCTCTC)TTTACCTTATTTATAGCCCAAGATTTCCTGGTTCAAGAATGGCCTGGACCTGGGAGAAGACGCCCGCTTCCGCATGTTCAGCAAGCAGGGAGTGTTGACT NGG 10 8 17 8 100 0 100 5 10
    251127162816_g2lh ATGCAGATTATTGAGGAATTTTCTAAAGGTATCTCTCTCGGTGTATTTCTCTACTTACCTGTAATAATGCTTTTGTCTTAATAGGGTGGTTCTCTTCCCAAAGTGGAAGCCAAATTCATCAATTATGTGAAGAATTGC(+ggcccctcccgcctcgaagaggagctccgccgccgcctcaccgaacccgga)TTCCGGATGACTGACCAAGAGGTAACTGGATTTTCTGGGGACATGATTAAATCCAAGTTTTTTTGTGATTTATTATGATTCTGCCTTTACCCTTTTTAAGTGTTGGCTCTTTTTAAAAA NGG 10 8 17 8 100 0 100 5 10
    251127161550_z5ne CCTCCATGCAGATGCAGCTGTACCTGCAGCAGCTGCAGAAGGTGCAGCCCCCTACGCCGCTACTCCCTTCCGTGAAGGTGcagtcccagcccccaccccccctgccgcccccaccccacccctct(+ggcccctcccgcctcgaagaggagctccgccgccgcctcaccgaacccgga)gtgcagcagcagctgcagcagcagccgccaccacccccaccaccccagccccagcctccaccccagcagcagcatcagccccctccacggcccgtGCACTTGCAGCCCATGCAGTTTTCC NGG 10 8 17 8 100 0 100 5 10
    251127160339_894c CGGGCCCATGAGGAGGCACGTCGgcgccaggagcagcagcagcagcagcgccaggagcaacagcagcagcagcaacagcaagcagcTGCGGTGGCTGCCGCC(+ggcccctcccgcctcgaagaggagctccgccgccgcctcaccgaacccgga)GCCACCCCACAGGCCCAGAGCTCCCAGCCCCAGTCCATGCTGGACCAGCAGAGGGAGTTGGCCCGGAAGCGGGAGCAGGAGCGAAGACGCCGGGAAGCCGTGA NGG 10 8 17 8 100 0 100 5 10
    251127160100_90mt GGGCCCATGAGGAGGCACGTCGgcgccaggagcagcagcagcagcagcgccaggagcaacagcagcagcagcaacagcaagcagcTGCGGTGGCTGCCGCC(+GGC)GCCACCCCACAGGCCCAGAGCTCCCAGCCCCAGTCCATGCTGGACCAGCAGAGGGAGTTGGCCCGGAAGCGGGAGCAGGAGCGAAGACGCCGGGAAGCCGTGAG NGG 10 8 17 8 24 0 100 5 10
    251126113722_g003 agaacctgaagcaccagccgggaggcgggaaggtgcagataattaataagaagctggatcttagcaacgtccagtccaagtgtggctcaaaggataatatcaaacacgtcccgggaggcggcagtgtgcaaatagtctacaaaccagttgacctgagcaaggtgacctccaagtgtggctcattaggcaacatccatcataaaccaggaggtggccaggtggaagtaaaatctgagaagcttgacttcaaggacagagtccagtcgaagattgggtccctggacaatatcacccacgtccctggcggagga(-AAT)aaaaagattgaaacccacaagctgaccttccgcgagaacgccaaagccaagacagaccacggggcggagatcgtgtacaagtcgccagtggtgtctggggacacgtctccacggcatctcagcaatgtctcctccaccggcagcatcgacatggtagactcgccccagctcgccacgctagctgacgaggtgtctgcctccctggccaagcagggtttg NGG 10 8 17 8 24 0 100 5 10
    251122011630_lppc tacttaatttttttttttttttttgcaggggtgagcacagacaggatcgcagggagaggct(/c)gtattaattagcctggctccccaggttctggaacagctttttgtcctgtacccagtgtta NGG 10 8 17 8 24 0 100 5 100
    251122011421_khry tacttaatttttttttttttttttgcaggggtgagcacagacaggatcgcagggagaggct(/c)gtattaattagcctggctccccaggttctggaacagctttttgtcctgtacccagtgtta NGG 10 8 17 8 24 0 100 5 10
    251120053213_rv02 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    251120053030_s1up CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10