Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    260929103136_h4ds GTTTCATCTGGAAAACCTGAGCCT(/A)GAGGATCCTGAAGAGCAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    260929103121_lx3u AGTTTCATCTGGAAAACCTGAGCC(/G)TGAGGATCCTGAAGAGCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    260929090006_nyhk AAGGCTGGTACTTTATAAGCCTGG(/A)AGGAAAATGTTTCAGTCCAGCAC NGG 10 8 17 8 24 0 100 5 10
    260928160600_ttrr GCTGGGATCAGGCAAGAGCCCCGCTCTCCTGGGCGCAGCTGCAGCCACCCAAGTCGTGGCCACAGAGCCAGGTGTCCCTGCACTCTCAGGGGCCCTGGTCCAAACCCAAGCCTCCAGGTGATCTTGCAAGCAGGGAGCACGCAGGAGACCCACCCTCCCGGGCACAGCTGCAGCTACCCAAGTTGTGGCTGTGGACCCAGGCATCCCTGCACTCTCAGGGTCTGGGAAGATCCCCCCTTGCCTCCGCAGGCT(/A)CAGAGCTGTCTGTTCCCACTGCTTGGCCTCTCCCCTGCTTCTGGCACCCGATCTGATCCTGGAGGCACAGTCGGGGCTACGCACTCTGTGGATCCAGTGTGAGTCGGGGACAAGCGGGACCCCTGCCCCTTCCGAGGTAGCCAGGAGCTCTCCTGGGGCAGCTGCAGCTGCCCAAGCCAGGACCCAGGCATCTCTACTGCCCCCACAAGCTCAGGGGTGTCTGCTCCCACTGTCTG NGG 10 8 17 8 24 0 100 5 10
    260928160518_xd1m AGCTGTCCCCCGTGACATCCAGAAAACGCGAAACCTCAGGAACAAGGTCGCAGCTTCAGACCGCGGCCCAGGAGGCCGATGGTGGGTGAGTGGGAGAGTCCCGGAGAGCAGGGGGGCAGAGAGCTGGTTTTCGGGAAAACCAATGTGTTGGACCCCAAACATCCACCCTCCGCTCGGATCCAAGTTCTCTGAGAACTGAAACGACATCCCGGGACGAATGGGAGAGTTAGGCTGAGCTACACACCGGGGAGGGGAGGGTTGGAG(/A)TTTAGCCCCAAGCCCTTCGGACGCCTTCTTCGGCTCCCGCGTGGGTTGAGACGGCGGCACGGCCACCAGACTCAGCTAAAGGGCGGAGTCGCGAGGAGAAGCCAGTGGCGAGGGGAGGAGGAGGCCTGGATCTCCCCGCGAAGGCTCCAGTCCGGCTTTTGCCTCCGACTGCGGGCTCCCTCCCCACCCGCCGTCCCTCGCCCCGCCCCGCC NGG 10 8 17 8 24 0 100 5 10
    260928090200_4byl AGTTGTCGACGGAGGCAGACATGGCATTATCTTATTCCGGTCCACCACGACCTTCCCGACGGGCCGCGGCGGCACACATTTGATCCCCCCCTCCCCCACA(/G)TGGCACCGCACCTCACCCCTCCACCACTCCCTCCTCCCGTCCTCCTCCCGTCTCGCCGTCGCCTCCTGCTCCTCCTCTTCCGCCGCCGACGTCGCCGCTAGCTGCCGCTTTTC NGG 10 8 17 8 24 0 100 5 10
    260927162317_baxk GGCCTCTGCAAGAAGCGCCCGAAGCCTGGAGGATGGAACACTGGGGGCAGCCGATACCCGGGGCAGGGCAGCCCTGGAGGCAACCGCTACCCACCTCAGGGCGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCTCATGGTGGTGGCTGGGGGCAGCCCCATGGTGGTGGCTGGGGACAGCCTCATGGTGGTGGCTGGGGTCAAGGAGGTGGCACCCACAGTCAGTGGAACAAGCCGAGTAAGCCAAAAACCAACATGAAGCACATGGCTGGTGCTGCAGCAGCTGGGGCAGTGGTGGGGGGCCTTGGCGG(/T)CTACATGCTGGGAAGTGCCATGAGCAGGCCCATCATACATTTCGGCAGTGACTATGAGGACCGTTACTATCGTGAAAACATGCACCGTTACCCCAACCAAGTGTACTACAGGCCCATGGATGAGTACAGCAACCAGAACAACTTTGTGCACGACTGCGTCAATATCACAATCAAGCAGCACACGGTCACCACAACCACCAAGGGGGAGAACTTCACCGAGACCGACGTTAAGATGATGGAGCGCGTGGTTGAGCAGATGTGTATCACCCAGTACGAGAGGGAATCTCAGGCCTATTA NGG 10 8 17 8 24 0 100 5 10
    260927162032_l864 GAGCTGCATCCCCGAGGGCCCGGTTCTCCAGCAGGCGCAGAGAGAGCAGGACGTCACAGTGACCGAGGGCCTGGAAGTTCGCTAATCCCGGAACTGGACCCCGCCCAAAGCCGCCCTCTTGCCTCCACTGGTTGTGCAGCCGCCGCTCCAGAGCCGTGCGAATGGGGCCATGCCGACCAAAGCGCCGATGGATGTGGCGCAGGTAGCGCGCCCACTGCAAGGCCCGGCGCACGGTGGCGGGGTCCCAGGTGCTGACGTAGGTAGTGCTTGAGACCGCCAGAAGCTCGGAAAAGCGATCCAC(/G)GTGCTGCAGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGACGGCGACTCTCTGCGTACTGATTGGAACATCCGCGAAATGATACGCCTCTCTGCAATGCTATTGGTCGAAATGCATGTCAATCTCCCAGCGTCTTTATCCGTGTTCCTTGACTCTGGGCAACTTAAAAGCCCTAATACTTTTACTTTCGCCACACAAAGAGGTTCTTCTTAGTGGAGGGAGAGCAGATGTAGGGCATCCTACCGAGAATTTCCGGAACCACGTGCGAGATGATGCCAGTCATGAACGTCTCCGCGCTTC NGG 10 8 17 8 24 0 100 5 10
    260927002318_9exv AATTGATGAATCAGTGCTGGAGAATGGGTCACAGTGGCAAATGAGGCTGGAGAGGCCCGTGAGCTGCTGTCCCAGAGGGCCTGGGATGTCCTGCAGGGAGCTTGGCATGAGAAACCTTGGAGAGTTTTAAGCAAGGGCTGATGTGGGCTGCCTAGAAAGGCATGGATGAGAGAAGCCTGGAGACAGGGATCCCAGGGAAACGCCCATGCAATTAGTCTATTTCTGCTGCAAGTAAGCATGCATTTGTAGGCTTGATGCTTTTTTTCTGCTTCTCCAGCCCTGGCCTGGGTCAATCCTTGGGGCCCAGACTGAGCACGT(/G)GATGGCAGAGGAAAGGAAGCCCTGCTTCCTCCAGAGGGCGTCGCAGGACAGCTTTTCCTAGACAGGGGCTAGTATGTGCAGCTCCTGCACCGGGATACTGGTTGACAAGTTTGGCTGGGCTGGAAGCCAGCACCTAGGGAGGTCCCTGGAAGGGGCCAGCCTCACCAGGAGAGGAGGGACCTGGCCCTTCAGGGTCGAGCTCAACAGAGGAAAAGATCTCAGGGCACCCAGAGCCCAGTGGCTTTCAGCACCTGCATGAAAATCAGAGATCAACCAGATTACCCCATATTGCCCAAGAGAAA NGG 10 8 17 8 24 0 100 5 10
    260926220645_v1kw GCTGGGATCAGGCAAGAGCCCCGCTCTCCTGGGCGCAGCTGCAGCCACCCAAGTCGTGGCCACAGAGCCAGGTGTCCCTGCACTCTCAGGGGCCCTGGTCCAAACCCAAGCCTCCAGGTGATCTTGCAAGCAGGGAGCACGCAGGAGACCCACCCTCCCGGGCACAGCTGCAGCTACCCAAGTTGTGGCTGTGGACCCAGGCATCCCTGCACTCTCAGGGTCTGGGAAGATCCCCCCTTGCCTCCGCAGGCT(/A)CAGAGCTGTCTGTTCCCACTGCTTGGCCTCTCCCCTGCTTCTGGCACCCGATCTGATCCTGGAGGCACAGTCGGGGCTACGCACTCTGTGGATCCAGTGTGAGTCGGGGACAAGCGGGACCCCTGCCCCTTCCGAGGTAGCCAGGAGCTCTCCTGGGGCAGCTGCAGCTGCCCAAGCCAGGACCCAGGCATCTCTACTGCCCCCACAAGCTCAGGGGTGTCTGCTCCCACTGTCTG NGG 10 8 17 8 24 0 100 5 10