Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250408223948_uems AGAGGCCAAGGAGACCACCACCTCCTTGCCTTCTCAGCTCACAAGTCTTGAGATCCACTGCTCAACTATGCACTAGGAATCAAGAGTCTTACCATCCAGAACCATGACTCTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCAC(-GGG)TCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408223938_wmqc AGAGGCCAAGGAGACCACCACCTCCTTGCCTTCTCAGCTCACAAGTCTTGAGATCCACTGCTCAACTATGCACTAGGAATCAAGAGTCTTACCATCCAGAACCATGACTCTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCAC(-G)GGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408223923_iw5c AGAGGCCAAGGAGACCACCACCTCCTTGCCTTCTCAGCTCACAAGTCTTGAGATCCACTGCTCAACTATGCACTAGGAATCAAGAGTCTTACCATCCAGAACCATGACTCTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGAGGCGCTCTAGGGGAAGGTTGATTATGCAC(-GG)GTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408211431_3ox9 CGGTGTGTGTAAAGCCTCTCCGGTGTGTGTGAAGCGTCTCCGGTGTGTGTGAAGCCTCTC(+GCCACCATGGC)ACTCTCCTCGACTCTTCATCATCATGTCTCTCCATCTCCTCCTCTTCTTCTTCCTCCAGCTCTTCAGCTCGTCTCTCCAGCAGTTCCCCCGAGTCTGCACCTCCCCAGAAGTCCTCCAGTCCA NGG 10 8 17 8 24 0 100 5 10
    250408204537_plnd GCGGCGTTTCTGCCTTGGCATCGGGTGTATCTGCTGTTTTGGGAGCATGAGATCCGGAAGCTGACGGGAGACTTTAACTTCACCATCCCGTACTGGGACTGGCGGGACGCGCAGGACTGT(+TGA)CAGGTGTGCACGGATGAGCTGATGGGGGCGCGCAGCAGCCTCAACCGCAGCCTGATCAGCCCCTCCTCTGTGTTCT NGG 10 8 17 8 24 0 100 5 10
    250408203951_agaa ACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCGA(-GG)CGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203824_bm0c ACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCCG(-AG)GCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203751_7gnn AGAGGCCAAGGAGACCACCACCTCCTTGCCTTCTCAGCTCACAAGTCTTGAGATCCACTGCTCAACTATGCACTAGGAATCAAGAGTCTTACCATCCAGAACCATGACTCTTCTTACTGAGGACCAGCCTTGCAGGTTCTCTGCACCGGAAAGCCCAATTGCATCCATGGAGCAGTACCAGGTGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTCC(-GA)GGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203716_7ii7 TGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCTC(-CG)AGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGACTTCCCAGACGTCTGTACAGTCTGGTGTGGCTCATAAGCCCCATTTTGGGTTTTATCC NGG 10 8 17 8 24 0 100 5 10
    250408203650_euk8 TGGACTCGGAGCTTAAAGAGGACTGTTTGGACAACATGGAAGCGGCTGTTTTTGGAGTGGTGGAGCCT(-CC)GAGGCGCTCTAGGGGAAGGTTGATTATGCACGGGTCGGCCATGTTTGGAAGGGAATTCTGCTACGCTGTTGAGGCTGCGTTTGTCACGCCAGTGTTGCTGAGCGTTGGAC NGG 10 8 17 8 24 0 100 5 10