Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    250403172834_lkil AAGTCTACTATCTTAAGTTTTGCAGTGGAGCGTCAACTAAACTTCTGTCCAGGAAAAGTTGTGTACTGAAATTAAGTGCTTTTGATAGTGGATCTATAGATCTCACCTCCTGCACCTATAGCTAATACACATTTGTCTTGCG(+ATG)TCATGGCTGGTAGCAGAAATCACTTTTGGATTATATTTTCATCTGCCACGTCTGCTTTATTGGCCTTTTTCCTGCTTTTCTGGGGGCCTTTCTGTGTGCAAAGTAGCCCCATCAGCTCCCCGGAGAGG NGG 10 8 17 8 24 0 100 5 10
    250403172831_fdmx AAGTCTACTATCTTAAGTTTTGCAGTGGAGCGTCAACTAAACTTCTGTCCAGGAAAAGTTGTGTACTGAAATTAAGTGCTTTTGATAGTGGATCTATAGATCTCACCTCCTGCACCTATAGCTAATACACATTTGTCTTGCG(+ATG)TCATGGCTGGTAGCAGAAATCACTTTTGGATTATATTTTCATCTGCCACGTCTGCTTTATTGGCCTTTTTCCTGCTTTTCTGGGGGCCTTTCTGTGTGCAAAGTAGCCCCATCAGCTCCCCGGAGAGG NGG 10 8 17 8 24 0 100 5 10
    250403172442_ueg0 AAGTCTACTATCTTAAGTTTTGCAGTGGAGCGTCAACTAAACTTCTGTCCAGGAAAAGTTGTGTACTGAAATTAAGTGCTTTTGATAGTGGATCTATAGATCTCACCTCCTGCACCTATAGCTAATACACATTTGTCTTGCGTC(+ATGGC)ATGGCTGGTAGCAGAAATCACTTTTGGATTATATTTTCATCTGCCACGTCTGCTTTATTGGCCTTTTTCCTGCTTTTCTGGGGGCCTTTCTGTGTGCAAAGTAGCCCCATCAGCTCCCCGGAGAGG NGG 10 8 17 8 24 0 100 5 10
    250403172224_ria1 AAGTCTACTATCTTAAGTTTTGCAGTGGAGCGTCAACTAAACTTCTGTCCAGGAAAAGTTGTGTACTGAAATTAAGTGCTTTTGATAGTGGATCTATAGATCTCACCTCCTGCACCTATAGCTAATACACATTTGTCTTGCGTC(+GCCACC)ATGGCTGGTAGCAGAAATCACTTTTGGATTATATTTTCATCTGCCACGTCTGCTTTATTGGCCTTTTTCCTGCTTTTCTGGGGGCCTTTCTGTGTGCAAAGTAGCCCCATCAGCTCCCCGGAGAGG NGG 10 8 17 8 24 0 100 5 10
    250402203712_3wp6 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250402110539_v568 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250402110508_u9dk CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250401231110_3yz2 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250401211025_wchh CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    250401195332_jokh CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10