Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241122114226_6yjc TGGGAACTCAGTTTATATGAGTTACAACGAACACCTCAGGTAATGACTAAGATGACT(/G)GCCAAGGGGCATATGAGACGTGTAAACTGGGATACATTTTTAG NGG 10 8 17 8 24 0 100 5 10
    241122113933_hnmb TGGGAACTCAGTTTATATGAGTTACAACGAACACCTCAGGTAATGACTAAGATGACT(/G)GCCAAGGGGCATATGAGACGTGTAAACTGGGATACATTTTTAG NGG 10 8 17 8 24 0 100 5 10
    241122113807_z0qq TGGGAACTCAGTTTATATGAGTTACAACGAACACCTCAGGTAATGACTAAGATGACTGCC(-AAG)GGGCATATGAGACGTGTAAACTGGGATACATTTTTAG NGG 10 8 17 8 24 0 100 5 10
    241122113317_dyai AAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCAC(-ATC)AACCGGTGGCGCATTGCCACGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    241122113113_2zma AAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCAC(-ATC)AACCGGTGGCGCATTGCCACGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    241122113042_4zuq AAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCAC(-ATC)AACCGGTGGCGCATTGCCACGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    241122113021_muth AAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCAC(-ATC)AACCGGTGGCGCATTGCCACGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    241122112609_3d77 AAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCAC(-ATC)AACCGGTGGCGCATTGCCACGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    241122112208_fpda AAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCACATC(-AAC)CGGTGGCGCATTGCCACGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    241122112117_q7ca AAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCACATC(-AAC)CGGTGGCGCATTGCCACGAAGCAG NGG 10 8 17 8 24 0 100 5 10