Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    241118105113_wk17 CCCTGGCCCAGGTGAAGGTGTGGTTCCAGAACCGGAGGACAAAGTACAAACGGCAG(+T)AAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGGGCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAATGGGGAGGACATCGATGTCACCTCCAATGACTAGGGTGGGCAACCACAAACCCACGAGGGCAGAGTGCTGCTTG NGG 10 8 17 8 24 0 100 5 10
    241118103005_550v AAGAACAATTTTATCCTCATCCTTGGAAGAATCCATTACCCTATCAA(-A)GTAATTTGGATGTTAATTTATGTTTGGATAAGACTTGTTTATCC NGG 40 8 17 8 40 0 100 5 10
    241117185942_cpje CACTGCAAGGCCCGGCGCACGGTGGCGGGGTCCCAGGTGCTGACGTAGGTAGTGCTTGAGACCGCCAGAAGCTCGGAAA(-A)GCGATCCAGGTGCTGCAGAAGGGATTCCATGAGGTGCGCGAAGGCCCTACTTCCGCTTTCACCTTGGAGA NGG 10 8 17 8 24 0 100 5 10
    241117183755_3t6g AGGTGAAGGTGTGGTTCCAGAACCGGAGGACAAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGG(+T)GCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAATGGGGAGGACATC NGG 10 8 17 8 24 0 100 5 10
    241117183711_qzaz AGGTGAAGGTGTGGTTCCAGAACCGGAGGACAAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGG(+T)GCTCCCATCACATCAACCGGTGGCGCATTGC NGG 10 8 17 8 24 0 100 5 10
    241117180734_ei58 GCCCAGGTGAAGGTGTGGTTCCAGAACCGGAGGACAAAGTACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGCAGAAGAAGAAGG(+T)GCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAATGGGGAGGACATCGATGTCAC NGG 10 8 17 8 24 0 100 5 10
    241116225217_tyhi cttcagaaggagaggaagttcctcaagacaaggcgccaagtcatgttcc(/t)tttcctgctaattggtggaggcacagctgcttttgctgcagccagatccatccgggctcgggatcctggggccagg NGG 10 8 17 8 24 0 100 5 10
    241116224804_z0ib cttcagaaggagaggaagttcctcaagacaaggcgccaagtcatgttcctttcc(/t)tgctaattggtggaggcacagctgcttttgctgcagccagatccatccgggctcgggatcctggggccagg NGG 10 8 17 8 24 0 100 5 10
    241116224725_1vaw cttcagaaggagaggaagttcctcaagacaaggcgccaagtcatgttcc(/t)tttcctgctaattggtggaggcacagctgcttttgctgcagccagatccatccgggctcgggatcctggggccagg NGG 10 8 17 8 24 0 100 5 10
    241114100739_pjyv cagcatggccccgctcagacccaagaaggccatggccggagcagcgcaggctctcctcccccaggcctgaccgactctgccggtggctccttccagttccccgaggtcgtccccctcaacattggaggggcgcacttcaccacacgcctgtccaccctgcggcgctacgaggataccatgctggcggcgatgttcagcgggcggcactacatccccaccgacgccgagggccgctacttcatcgaccgggacggcgcatacttc(+TA)gggtatgtgtctccctctgccaccctgctgcctcgtcttagtgagggagcggccgggcttggctgtgaggctttgatagcttccccaacagctggaggaggagataacctgctggagggatgaatacatgtgcatattgataagagttccctggtggctcagtggctttggggcctggcattgtcattgctgtggcttgtgttgcatccttggc NGG 10 8 17 8 24 0 100 5 10