Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240730051058_be6eb1em agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACA(+CAC)TCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10
    240730051058_be6eay67 agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCACCT(+ATG)CTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10
    240730051058_be6eavgt agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCACCTCTAAACTTCAAGAAGCGCCTG(+ATC)TTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10
    240730051058_be6eapf8 agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCACCTCTAAACTTCAAGAAGCGC(+CTG)CTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10
    240730051058_be6eao4d agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGA(+TGG)TCCCAACAGAAAAAGAAAACATCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10
    240730051058_be6eany3 agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCACCTCTA(+CAG)AACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10
    240730051058_be6eadw8 agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGA(+AAG)TCCCAACAGAAAAAGAAAACATCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10
    240730051058_be6eaces agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCA(+TGC)CCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10
    240730051058_be6ea9a9 agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCC(+CAG)GCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10
    240730051058_be6ea8p7 agcaataatagtaggctgagggggaaccaagagggatgaggattaatgtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctATGGCC(+GTG)GCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGACTTTGAACGTGGGgtaagtttctcgactatgaaaactgagtttcaagatatcaaggacttggccttagatctttcttggggaagaggtaaattttcgttggtaggaggagggg NGG 10 8 17 8 24 0 100 5 10