Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613124014_vjvi GACCCCGTGGGTGAACACGCACCAGGGTGGGG(+AGGACA)GTACCAGTGCTCCAGTTGGCCTACTTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613124012_vg1w GAAGGGGAAGAAGTAGGCCAACTGGAGCACTG(+AGGACA)GTACCCCCACCCTGGTGCGTGTTCACCCACG NGG 10 8 17 8 24 0 100 5 10
    240613124011_on4j TCTTGCTGAAATAAAGGTAAAGTCCACATTTG(+AGGACA)GATCCTTTGCTCCTGCACCACTTTTCCATTG NGG 10 8 17 8 24 0 100 5 10
    240613124010_9pls CCAGCCAATGGAAAAGTGGTGCAGGAGCAAAG(+AGGACA)GATCCAAATGTGGACTTTACCTTTATTTCAG NGG 10 8 17 8 24 0 100 5 10
    240613124007_eetw TTTTTCTCTGAAATTCCTTGTCTCCGCCTCCG(+AGGACA)GATCCAGGTCAGCAGCAGCCCCCAGAGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613124006_kllb CAGCCACAGCTCTGGGGGCTGCTGCTGACCTG(+AGGACA)GATCCGGAGGCGGAGACAAGGAATTTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240613124003_pzdt TCAGCCTGAACCATTTCTCATCTGTTTCCCAG(+AGGACA)GATCCAAAATTGTACCTGCCTTTCAATAACT NGG 10 8 17 8 24 0 100 5 10
    240613124002_7dfz GGAGGAGTTATTGAAAGGCAGGTACAATTTTG(+AGGACA)GATCCTGGGAAACAGATGAGAAATGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240613124000_kgat GAGGTCTTAAGATCCCATGGAGGGTCCTGTGG(+AGGACA)GTACCTTGTGGGGGTCCGCAGACTCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240613123959_pylt CCTTGGGCACAGAGTCTGCGGACCCCCACAAG(+AGGACA)GTACCCACAGGACCCTCCATGGGATCTTAAG NGG 10 8 17 8 24 0 100 5 10