Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035956_xvxh GCTTAAGATTTTGCTACAGAGACAAAAAGGTG(+ATATAT)GATCCTTTCCCTGCCCAGCTACTCTGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240607035955_uh3x CCCTGGTGGACAGAGTAGCTGGGCAGGGAAAG(+ATATAT)GATCCACCTTTTTGTCTCTGTAGCAAAATCT NGG 10 8 17 8 24 0 100 5 10
    240607035954_qba6 AGTGGTTAAGAGCACTGACTGTTCTTAGAGAG(+ATATAT)GATCCAGGTTTGATTCCCAACACCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240607035952_bdu0 GCTTTCCATGTGGGTGTTGGGAATCAAACCTG(+ATATAT)GATCCTCTCTAAGAACAGTCAGTGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240607035951_tvj8 ATTCTGCCATGTGGGTGCTTACCTGTGCTGAG(+ATATAT)GTACCAAGCACCTGTCTAGTTACATCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240607035950_y8ey TGCCCAGGAAGATGTAACTAGACAGGTGCTTG(+ATATAT)GTACCTCAGCACAGGTAAGCACCCACATGGC NGG 10 8 17 8 24 0 100 5 10
    240607035949_k454 GGTGGAGACTAATATAGCTGTCTCATTTAGAG(+ATATAT)GTACCCATTCAGTCTCTATAGCCCTTTGACC NGG 10 8 17 8 24 0 100 5 10
    240607035947_nobw CAACTGGTCAAAGGGCTATAGAGACTGAATGG(+ATATAT)GTACCTCTAAATGAGACAGCTATATTAGTCT NGG 10 8 17 8 24 0 100 5 10
    240607035946_l9bz GTCCACTTCTCCATGTATTGAGGTGATCAGTG(+ATATAT)GATCCTTGAATATCTATATTTTCTACAATTA NGG 10 8 17 8 24 0 100 5 10
    240607035944_8jj9 ATCACTAATTGTAGAAAATATAGATATTCAAG(+ATATAT)GATCCACTGATCACCTCAATACATGGAGAAG NGG 10 8 17 8 24 0 100 5 10