Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005610_svft TCTGAGAGCCAGATGTTAAGACAAGCTGGTGG(+ATATAT)GATCCCCACTTTGTGAGAGAAGGGAGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607005608_k8fk AGGGGCCTTTCTCCCTTCTCTCACAAAGTGGG(+ATATAT)GATCCCACCAGCTTGTCTTAACATCTGGCTC NGG 10 8 17 8 24 0 100 5 10
    240607005606_6ljq ACATATATGTCTGTGTCCTACATGCATGCCTG(+ATATAT)GTACCCACAGAAGCCAGAAGAGGGCATTAGG NGG 10 8 17 8 24 0 100 5 10
    240607005604_c505 GAGGACCTAATGCCCTCTTCTGGCTTCTGTGG(+ATATAT)GTACCAGGCATGCATGTAGGACACAGACATA NGG 10 8 17 8 24 0 100 5 10
    240607005602_xcxd AAGGGCCGACACAGTGCAAGCAGGGGGCCCTG(+ATATAT)GATCCTACCCAGATCCCATCCCATCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607005600_x8hj CCAGAGCTGGGGATGGGATGGGATCTGGGTAG(+ATATAT)GATCCAGGGCCCCCTGCTTGCACTGTGTCGG NGG 10 8 17 8 24 0 100 5 10
    240607005559_x34r TCTACAAAACTCCATGGAGCCATGAAATTGGG(+ATATAT)GTACCTAAATCTATGCTTCTAGGGCCATGGT NGG 10 8 17 8 24 0 100 5 10
    240607005557_8w9f CAGTGACCATGGCCCTAGAAGCATAGATTTAG(+ATATAT)GTACCCCAATTTCATGGCTCCATGGAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240607005556_f4on TGGAGTAGGGAAAAGGCCGACTCAGCCTAACG(+ATATAT)GATCCCCCTGAGTCCCAGACAAAAGCCACTC NGG 10 8 17 8 24 0 100 5 10
    240607005554_sz7i CAACTGAGTGGCTTTTGTCTGGGACTCAGGGG(+ATATAT)GATCCGTTAGGCTGAGTCGGCCTTTTCCCTA NGG 10 8 17 8 24 0 100 5 10