Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606221609_ji00 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606221607_1gby CTTGTACTTTTCTTTATTCTCTACAAGACTGG(+ATATAT)GATCCATCTTGGAAACATTAAGAATATCTAC NGG 10 8 17 8 24 0 100 5 10
    240606221606_1ica ATCCAGTAGATATTCTTAATGTTTCCAAGATG(+ATATAT)GATCCCAGTCTTGTAGAGAATAAAGAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606221604_3jj4 AATGTCTGCGTTTGGTGGCTGATTATGGGATG(+ATATAT)GATCCCCGGATGGGGCAGTCTCTGGATAGTT NGG 10 8 17 8 24 0 100 5 10
    240606221602_claf ACTGTAACTATCCAGAGACTGCCCCATCCGGG(+ATATAT)GATCCATCCCATAATCAGCCACCAAACGCAG NGG 10 8 17 8 24 0 100 5 10
    240606221600_yuhp AAGCATGGACCAACTTAAAGGGTTTGCACTGG(+ATATAT)GATCCTCTGTGGAGCTGAGTTCTACCACCTT NGG 10 8 17 8 24 0 100 5 10
    240606221559_hvxk GTCAGAAGGTGGTAGAACTCAGCTCCACAGAG(+ATATAT)GATCCCAGTGCAAACCCTTTAAGTTGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240606221556_j3q0 GCAGACAGTGTATCTGAAAAGTCTCTTCCCAG(+ATATAT)GATCCAGAATGGGATGAACCTGTCTTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606221555_o06d GAGGGAGCACAAGACAGGTTCATCCCATTCTG(+ATATAT)GATCCTGGGAAGAGACTTTTCAGATACACTG NGG 10 8 17 8 24 0 100 5 10
    240606221553_es18 AAGTGTTGTTGGGCAGTCCCGTGTCATCCATG(+ATATAT)GTACCTGCTTCAATTTCACACATAGCTGGTA NGG 10 8 17 8 24 0 100 5 10