Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606181937_k3vk ATTCAGAGGCAAAGAATCAGACGGCTCATGTG(+AAAAAA)GATCCATTTCTGATTCCTGTCACTTCTCCAC NGG 10 8 17 8 24 0 100 5 10
    240606181935_van3 CCAGTGCTCTGAACAATATGTCAAGTGCTTGG(+AAAAAA)GATCCGCTTTTAAGATGAATTTCTTGAGCAT NGG 10 8 17 8 24 0 100 5 10
    240606181934_3qob TTAATATGCTCAAGAAATTCATCTTAAAAGCG(+AAAAAA)GATCCCAAGCACTTGACATATTGTTCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606181933_q1xe AGTTTGACCCCAACACAAAGGGGAAAGACAGG(+AAAAAA)GTACCTTTCTTCATGAGTTGTCTCCCTACCC NGG 10 8 17 8 24 0 100 5 10
    240606181931_pors GGGGGGGGTAGGGAGACAACTCATGAAGAAAG(+AAAAAA)GTACCCTGTCTTTCCCCTTTGTGTTGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606181929_gnrx TGGAGAGGCCAGGACCCCACCTGCTGCATGGG(+AAAAAA)GATCCACTAGCCATGGAAGGATACAATAGGA NGG 10 8 17 8 24 0 100 5 10
    240606181928_nd2v AGGCTTCCTATTGTATCCTTCCATGGCTAGTG(+AAAAAA)GATCCCCATGCAGCAGGTGGGGTCCTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606181927_uwaq TTCCATTCAGAAGAATGGGACACAGCTGACAG(+AAAAAA)GATCCAGTGCTTGCTAGATTTCATCAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240606181926_c71v TCTGAAGAACTGATGAAATCTAGCAAGCACTG(+AAAAAA)GATCCTGTCAGCTGTGTCCCATTCTTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606181925_w0fl TACATTATAAGCTATTTTCTGATTCTGTAATG(+AAAAAA)GTACCCTTGCTCTCATTTTAAAGAAGTTTTT NGG 10 8 17 8 24 0 100 5 10