Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606153435_n4q5 GTAGGGGTGGCTAACAAGCAGGAATAGGAAGG(+AAAAAA)GATCCTTGGTAGCTTGTTTTTTGTCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606153433_rfpa AGATCCCAACAGACAAAAAACAAGCTACCAAG(+AAAAAA)GATCCCTTCCTATTCCTGCTTGTTAGCCACC NGG 10 8 17 8 24 0 100 5 10
    240606153432_vktv AAGTCACAGGTTGGTAGGTGTCATTGTCATTG(+AAAAAA)GATCCTAGCTGACAGACAGATGAGGGGGAAT NGG 10 8 17 8 24 0 100 5 10
    240606153430_krmc AGTGGATTCCCCCTCATCTGTCTGTCAGCTAG(+AAAAAA)GATCCAATGACAATGACACCTACCAACCTGT NGG 10 8 17 8 24 0 100 5 10
    240606153429_9beg AGCCTACCCCTGACACCGCCTGCAGGGCCAAG(+AAAAAA)GATCCAAAGCCTGGAAAGGAGAACCAAACAT NGG 10 8 17 8 24 0 100 5 10
    240606153428_ngbx CAGGGATGTTTGGTTCTCCTTTCCAGGCTTTG(+AAAAAA)GATCCTTGGCCCTGCAGGCGGTGTCAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240606153426_s59l GAATAATTTTTGGGACGTGGAAATTGCTAGAG(+AAAAAA)GTACCTTTTCTGTAACAATGAGAAGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240606153425_rd7m TCCTAGAAGCTCTTCTCATTGTTACAGAAAAG(+AAAAAA)GTACCTCTAGCAATTTCCACGTCCCAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240606153423_r3fr GGCATTTTCCTAATTAGTGATTGATAGGGGAG(+AAAAAA)GATCCAGCCATTTGTGGGTGGTGCCATCCCT NGG 10 8 17 8 24 0 100 5 10
    240606153422_wxrc AGCCAAGGGATGGCACCACCCACAAATGGCTG(+AAAAAA)GATCCTCCCCTATCAATCACTAATTAGGAAA NGG 10 8 17 8 24 0 100 5 10