Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606142012_ka66 GTCATATATCGTAAATATTTCTCTTCTCAAAG(+AAAAAA)GATCCAATAACCAGTTAAGTCCAAGCAATAG NGG 10 8 17 8 24 0 100 5 10
    240606142011_qot5 TTAAGTACTTCATAACCTATTAAGGAAAACAG(+AAAAAA)GTACCTGGTGTCTCAGGAAGGCAATCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240606142009_usrd GTGTGATCTTGATTGCCTTCCTGAGACACCAG(+AAAAAA)GTACCTGTTTTCCTTAATAGGTTATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606142006_z6nc CTGGAAGGAGTTAGCTCCCAGAGCTCCCCAGG(+AAAAAA)GTACCCTCCCGGGGTCGGCCTTACCTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606142004_oxum TGGAGATGGAAGGTAAGGCCGACCCCGGGAGG(+AAAAAA)GTACCCTGGGGAGCTCTGGGAGCTAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240606142002_kqsj TTGAGTTATGGATCGTTAGATCTTCATCCTGG(+AAAAAA)GATCCAGTTCCATTTTCTCTTCCGTCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606142000_ssg1 TATCCTCAGGGACGGAAGAGAAAATGGAACTG(+AAAAAA)GATCCCAGGATGAAGATCTAACGATCCATAA NGG 10 8 17 8 24 0 100 5 10
    240606141959_3oqw GACAGTCCATCCATCCTTAACTGCAGAATCTG(+AAAAAA)GATCCCCTGCCCTCCAGTTTTGCTGCCGTTT NGG 10 8 17 8 24 0 100 5 10
    240606141957_fjs2 TTGATAAACGGCAGCAAAACTGGAGGGCAGGG(+AAAAAA)GATCCAGATTCTGCAGTTAAGGATGGATGGA NGG 10 8 17 8 24 0 100 5 10
    240606141956_ac65 ATATGTGTGTGTGTGTGTGTGTGTGTATATAG(+AAAAAA)GTACCTACAATCTTTGTGGAATAGATTCTAA NGG 10 8 17 8 24 0 100 5 10