Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606122309_i83m TCACAAAGGGGACCCCCTCCCAGGGCCTACAG(+AAAAAA)GATCCAGGGATTGAATGCTCAGGGAAGCACT NGG 10 8 17 8 24 0 100 5 10
    240606122306_qd5w GTGGTAGTGCTTCCCTGAGCATTCAATCCCTG(+AAAAAA)GATCCTGTAGGCCCTGGGAGGGGGTCCCCTT NGG 10 8 17 8 24 0 100 5 10
    240606122305_vykh ACTACCAAATTGCTTGGCCCTTACCCTTCCAG(+AAAAAA)GATCCCTGCATATCCTCTCCAGCCCCAACCC NGG 10 8 17 8 24 0 100 5 10
    240606122303_ugjn GGGGTGGGTTGGGGCTGGAGAGGATATGCAGG(+AAAAAA)GATCCTGGAAGGGTAAGGGCCAAGCAATTTG NGG 10 8 17 8 24 0 100 5 10
    240606122302_dbu9 TTGCTACTGGGCCACAGAATGGGTAACACGTG(+AAAAAA)GTACCAACTTTGATTTGGTCAAAGTTGATGT NGG 10 8 17 8 24 0 100 5 10
    240606122301_lhgj AGGCAACATCAACTTTGACCAAATCAAAGTTG(+AAAAAA)GTACCACGTGTTACCCATTCTGTGGCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240606122259_m8my TGCTACCCAAGGAGAGCATGCCAGATAGCGAG(+AAAAAA)GATCCAAGGTCAAGGCAGAGCCTTGACAGCT NGG 10 8 17 8 24 0 100 5 10
    240606122258_d8zk CTCTTAGCTGTCAAGGCTCTGCCTTGACCTTG(+AAAAAA)GATCCTCGCTATCTGGCATGCTCTCCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606122256_k4e8 CTGCTGTGGGTTCTGACAGTGACTGAGGCCTG(+AAAAAA)GATCCCCACCCTGCCCCAGCCTGAGAAAGGT NGG 10 8 17 8 24 0 100 5 10
    240606122254_svwh CATGGACCTTTCTCAGGCTGGGGCAGGGTGGG(+AAAAAA)GATCCAGGCCTCAGTCACTGTCAGAACCCAC NGG 10 8 17 8 24 0 100 5 10