Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605200755_x6ew ATCCCCCTTCCCATGCCCTGGTTCTCAGCTAG(+CAGTGT)GTACCTGCAGCTCTCTCTGTGTGTCAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605200753_rqab ATTATTATTATTATTACTTGTGTGTGTGTGTG(+CAGTGT)GATCCCACATCCTCCAACAGCTCCAGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605200752_frgz CTTCCAACCCCTGGAGCTGTTGGAGGATGTGG(+CAGTGT)GATCCACACACACACACAAGTAATAATAATA NGG 10 8 17 8 24 0 100 5 10
    240605200750_d3a0 CTCAAAGCTTTGAGGAGTGAAGTTAGGAGTAG(+CAGTGT)GTACCAGTGACTACGAACTGAACATGCACTG NGG 10 8 17 8 24 0 100 5 10
    240605200749_70p9 GCTCACAGTGCATGTTCAGTTCGTAGTCACTG(+CAGTGT)GTACCTACTCCTAACTTCACTCCTCAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605200747_fbhp GTAGTGATAAACTTCTGGTGGGATAGAAAGAG(+CAGTGT)GATCCATCTTCAAACCTCAAGTCTTCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605200746_lk4h TCCCATCCAGGAAGACTTGAGGTTTGAAGATG(+CAGTGT)GATCCTCTTTCTATCCCACCAGAAGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240605200745_123w ATTAAGAAGAGATTTTTTTAATCTATTGAATG(+CAGTGT)GATCCTCATTTGTAAAAATTTTTAGCCATTT NGG 10 8 17 8 24 0 100 5 10
    240605200743_rggq AACAAAAATGGCTAAAAATTTTTACAAATGAG(+CAGTGT)GATCCATTCAATAGATTAAAAAAATCTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605200741_40vo CCCCTGTCCTGCTGATGCTGTCTATCCCCTGG(+CAGTGT)GATCCAGGGTCCTCTGCCTGCCCTCCTTTGG NGG 10 8 17 8 24 0 100 5 10