Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605165213_q715 CCTTCAGTTTATGATGGCTACCTATATACATG(+CAGTGT)GATCCATAAAACCAATGCAAAAGGTAGACTT NGG 10 8 17 8 24 0 100 5 10
    240605165211_vitl CACGTGACCCTGAACAACAGTGAGCCTCTCGG(+CAGTGT)GATCCAGCCCACCATCACCTTGTTCGGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605165210_3a09 CTCATAGCTCCGAACAAGGTGATGGTGGGCTG(+CAGTGT)GATCCCGAGAGGCTCACTGTTGTTCAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605165208_objk TGTTATTTCTTTAGCAGTGGTTAGGATGCTAG(+CAGTGT)GTACCACTGGTTTCTCTCATCAACGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605165206_54ib TTTGGCTGCAGCGTTGATGAGAGAAACCAGTG(+CAGTGT)GTACCTAGCATCCTAACCACTGCTAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605165204_1y74 TTAACTACATCTACAGCTATGTTTGTTTCCCG(+CAGTGT)GTACCCGGTTACAGTGAGTCTCTGCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605165202_zaav TTCACAGCAGGGCAGAGACTCACTGTAACCGG(+CAGTGT)GTACCGGGAAACAAACATAGCTGTAGATGTA NGG 10 8 17 8 24 0 100 5 10
    240605165200_45t2 GCAGATGTTTTTGAGCTGCCATGTGATGACAG(+CAGTGT)GATCCAAACCAGAGTTCTCTGCAAGAGCGGC NGG 10 8 17 8 24 0 100 5 10
    240605165159_g88j CACTTGCCGCTCTTGCAGAGAACTCTGGTTTG(+CAGTGT)GATCCTGTCATCACATGGCAGCTCAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240605165158_flok TCTGTACCTAGGGTCTGAACTTAGTTAATGAG(+CAGTGT)GATCCCAGCAAGACTCTCCAGTTTACATTCC NGG 10 8 17 8 24 0 100 5 10