Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605163807_p5ou TATGTGTCCTTCTCTCTGGGACTGCATGTCTG(+CAGTGT)GATCCTTGGGGATGTCTTGTGAGTAGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605163805_w2lj GCCATTCCGAGCCTGGGACCTGACCCTGTGTG(+CAGTGT)GATCCCGTCCCCAGCCAACAGCAGAGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605163804_ljp0 GGCCTGGCAGCTCTGCTGTTGGCTGGGGACGG(+CAGTGT)GATCCACACAGGGTCAGGTCCCAGGCTCGGA NGG 10 8 17 8 24 0 100 5 10
    240605163802_3lax AACTGTTCTTGTCCTTGTCACTCAGGGCAGAG(+CAGTGT)GATCCTGGGCATACTGAGGAGACCATTGCTA NGG 10 8 17 8 24 0 100 5 10
    240605163800_0c79 GACAATAGCAATGGTCTCCTCAGTATGCCCAG(+CAGTGT)GATCCTCTGCCCTGAGTGACAAGGACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605163758_zrz9 AACTGTTCTTGTCCTTGTCACTCAGGGCAGAG(+CAGTGT)GATCCTGGGCATACTGAGGAGACCATTGCTA NGG 10 8 17 8 24 0 100 5 10
    240605163756_a97n GACAATAGCAATGGTCTCCTCAGTATGCCCAG(+CAGTGT)GATCCTCTGCCCTGAGTGACAAGGACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240605163753_7a3c AAGAATGTGTGTCATGGTAGAGGGGTTCCCAG(+CAGTGT)GATCCTCTCCTGCCAGATGCTTGGCTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605163752_uadz GACCTGCAGCAGCCAAGCATCTGGCAGGAGAG(+CAGTGT)GATCCTGGGAACCCCTCTACCATGACACACA NGG 10 8 17 8 24 0 100 5 10
    240605163749_m16r CACCTACCCCGATGCCCGAGTTCAACTCCCAG(+CAGTGT)GATCCACTTGGTGGAAGGAGACCACTGATCC NGG 10 8 17 8 24 0 100 5 10