Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605132549_20u8 TGGGAGGATCATTAGTTCAAGGCCAAACTAGG(+CAGTGT)GTACCTTGTGATAACCTTTCATAAATATAAG NGG 10 8 17 8 24 0 100 5 10
    240605132548_4qzi AATGTCTTATATTTATGAAAGGTTATCACAAG(+CAGTGT)GTACCCTAGTTTGGCCTTGAACTAATGATCC NGG 10 8 17 8 24 0 100 5 10
    240605132547_00bb TCAGGCCCAGCCAAAGCTACCAGAGCTCTAAG(+CAGTGT)GTACCTGGGGACCAATGGAATTTACACCTAT NGG 10 8 17 8 24 0 100 5 10
    240605132544_7rj8 TTACCATAGGTGTAAATTCCATTGGTCCCCAG(+CAGTGT)GTACCTTAGAGCTCTGGTAGCTTTGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605132543_uvga GAGAAACCAAAATGCTGGTGACCCTTAGAGAG(+CAGTGT)GATCCAGTCACCTTCCTTTAAGTCCTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605132541_qeev ACTGTGGAGAAGGACTTAAAGGAAGGTGACTG(+CAGTGT)GATCCTCTCTAAGGGTCACCAGCATTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605132540_3ixy GAAGCCTTCATGAGTCTTCCACTCTCTTCTGG(+CAGTGT)GTACCGACGCAGAGATGGCATGTCACAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605132538_msh6 CCGGAATCCTGTGACATGCCATCTCTGCGTCG(+CAGTGT)GTACCCAGAAGAGAGTGGAAGACTCATGAAG NGG 10 8 17 8 24 0 100 5 10
    240605132537_ef0e GTTACCTCACAGACCACCACAGCCCACTCTGG(+CAGTGT)GTACCATCATCCCTGTCTCTGCCATTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605132536_rrmm CTAGGGCAGGAATGGCAGAGACAGGGATGATG(+CAGTGT)GTACCCAGAGTGGGCTGTGGTGGTCTGTGAG NGG 10 8 17 8 24 0 100 5 10