Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605131351_lpbb ACGTGGGGCCCATTTTGTTGGGGGCAGCCTGG(+CAGTGT)GATCCCTCCATTTCTTGACATGTCCTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605131349_uswx CCTTTCTCCACAAGCAAGGCATGGCGTACCAG(+CAGTGT)GATCCCCACACTGTCCTGGCCTACTTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240605131347_5k12 TGAAGGTAGAAAGTAGGCCAGGACAGTGTGGG(+CAGTGT)GATCCTGGTACGCCATGCCTTGCTTGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605131346_qdsr GGACTCGAGTGGCTCACAACCATCTGTAATAG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605131345_c8lj GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCTATTACAGATGGTTGTGAGCCACTCG NGG 10 8 17 8 24 0 100 5 10
    240605131342_tsk7 ATACCAGGACCCACGTGTGCTAGTGTCCCTGG(+CAGTGT)GATCCCAGTTCACAGAAGAGCACCAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605131340_hgpx CTTCCAGGCCTTGGTGCTCTTCTGTGAACTGG(+CAGTGT)GATCCCAGGGACACTAGCACACGTGGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605131337_sre5 CCTTAAGCTTATCTGCCTATTGATATCCCAGG(+CAGTGT)GATCCTGAAACAGGCCCCTGCGCATACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605131336_u37y TGTTCCTCTGTATGCGCAGGGGCCTGTTTCAG(+CAGTGT)GATCCCTGGGATATCAATAGGCAGATAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605131334_gkq1 GGATTGCCAGCCTCCCTCAGCAGGGGCACTTG(+CAGTGT)GTACCCCCATAAGGGTGGCATGCCCCAGTCC NGG 10 8 17 8 24 0 100 5 10