Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605120743_z7kz AGAAAGGGCTGCAGTCAGTTTGACTAAAACGG(+CAGTGT)GATCCACGTTATTGCTTAGAAAGGAAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240605120742_jug3 GCTGGCTTGCTTCCTTTCTAAGCAATAACGTG(+CAGTGT)GATCCCGTTTTAGTCAAACTGACTGCAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605120740_pdvm AGTATAGTATCAATAACGTCTACATCACCAGG(+CAGTGT)GTACCTTTGCATTAAATGAGTTTTGCAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605120738_efp8 TTACTATTTTGCAAAACTCATTTAATGCAAAG(+CAGTGT)GTACCCTGGTGATGTAGACGTTATTGATACT NGG 10 8 17 8 24 0 100 5 10
    240605120737_1nio CAAAGATTAAAGAGGACCAAAAGATGAGTTCG(+CAGTGT)GTACCATAACAAAAAAAATCTCACCTACTGT NGG 10 8 17 8 24 0 100 5 10
    240605120736_dq35 ATGGTACAGTAGGTGAGATTTTTTTTGTTATG(+CAGTGT)GTACCGAACTCATCTTTTGGTCCTCTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240605120735_xe4p ACTGTCTGGGTTTGGTGACTGATAATGGAATG(+CAGTGT)GATCCCCAGTTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240605120733_wce3 GGAATGACCATCCAGAGACTGCTCTAACTGGG(+CAGTGT)GATCCATTCCATTATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605120731_insy TTCTGGGTATGTGTCCAGGAGCATAATTGCTG(+CAGTGT)GATCCTCCTGTAGTACTGTGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605120730_r2ea TACTCAGAAAATAGTTCACAGTACTACAGGAG(+CAGTGT)GATCCAGCAATTATGCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10