Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605115038_f1ip GAGTCAATGTCATGGTAGTCTGCAGGGGGAAG(+CAGTGT)GATCCCTTGGTACAAACTTCATGTCTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605115036_277a TTATCTTTAAAGACATGAAGTTTGTACCAAGG(+CAGTGT)GATCCTTCCCCCTGCAGACTACCATGACATT NGG 10 8 17 8 24 0 100 5 10
    240605115034_bxn0 AGAACCTTGTATTCCATACTGTCCTGGCCCAG(+CAGTGT)GTACCTCTGAGTAAGAAACCAGCTGGAGATT NGG 10 8 17 8 24 0 100 5 10
    240605115032_3pm9 TCCAAAATCTCCAGCTGGTTTCTTACTCAGAG(+CAGTGT)GTACCTGGGCCAGGACAGTATGGAATACAAG NGG 10 8 17 8 24 0 100 5 10
    240605115031_xmx3 ATGGCATAGTCCCTTCCCCTTGTGCTCTGTTG(+CAGTGT)GTACCTCTCTCAGGGACTGACCACTACCAGC NGG 10 8 17 8 24 0 100 5 10
    240605115030_wx37 TTGCTGCTGGTAGTGGTCAGTCCCTGAGAGAG(+CAGTGT)GTACCAACAGAGCACAAGGGGAAGGGACTAT NGG 10 8 17 8 24 0 100 5 10
    240605115029_1ygc ATCAATAGCCCCTCCACATCACCATAAAACTG(+CAGTGT)GATCCCTTTTTTTAAGCACCCTCCTATGGCT NGG 10 8 17 8 24 0 100 5 10
    240605115027_u1r3 TGGGAAGCCATAGGAGGGTGCTTAAAAAAAGG(+CAGTGT)GATCCAGTTTTATGGTGATGTGGAGGGGCTA NGG 10 8 17 8 24 0 100 5 10
    240605115024_hk7f GTGAAAGGTGCCAGGAGTGGGAGGCTCCCAAG(+CAGTGT)GATCCAAATGTGCGTGGTGCAGGCGCATGCC NGG 10 8 17 8 24 0 100 5 10
    240605115023_zseh GACTTGGCATGCGCCTGCACCACGCACATTTG(+CAGTGT)GATCCTTGGGAGCCTCCCACTCCTGGCACCT NGG 10 8 17 8 24 0 100 5 10