Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605045635_gfbi1it6 TGTAGAATATTTCAATTGCCAACAGGAGCTGGCTTCAGAGTTGAATAAACC(/T)GTATCAGATAGTAGAAAGAGTAATAGGTAAGTACATGGTAACTCACTTC NGG 10 8 17 8 24 0 100 5 10
    240605045635_gfbi0ntu TCAATTTGGCTTCAGCGGACACAGTCGTCATCTTTGACTCTGACTGGAACC(/G)CCCAGAATGACTTGCAGGCACAAGCCCGAGCGCATAGAATTGGTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240605045635_gfbi0jgw CGTGATGCTGAGCTGGTAGATAAGTCGGTGGCAGATCTGAAGCGCCTGGGG(/A)GAACTGATCCACAACAGCTGTGTGTCAGCAATGCAGGAATACGAAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605045635_gfbi0fw7 ATAGCTCATAGTCGGAAGCCGGCACCCTCAAATGAGCCCGAATATCTATGG(/T)AAATGGATGGGACTCCCCTATTCAGAGTGTAGCTGGGAAGATGAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240605035035_1stb CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240605032609_mkxm GCCAGGGTGAGGGGAAGCCGGCCAGGCTGGCG(+ACTCAC)GATCCGACCCGGCGAGGCGGTCGCGTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605032608_i9yt CCGATGGAAAACGCGACCGCCTCGCCGGGTCG(+ACTCAC)GATCCGCCAGCCTGGCCGGCTTCCCCTCACC NGG 10 8 17 8 24 0 100 5 10
    240605032606_fmrg TGAAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ACTCAC)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240605032604_4onk ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+ACTCAC)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605032603_p74x ATTGAAAAAAAATGTTTGTTTTCCTGTGTTGG(+ACTCAC)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10