Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604233430_ypmr GGGCCCAGTCCCAGTGCCCCAGGACTCCTGTG(+ACTCAC)GATCCAACCAGCTTCCTCTCCCCCGGGACTA NGG 10 8 17 8 24 0 100 5 10
    240604233429_74bg AACTTTAGTCCCGGGGGAGAGGAAGCTGGTTG(+ACTCAC)GATCCACAGGAGTCCTGGGGCACTGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240604233427_avpc AAACCCCTGGTGCTCTCCCAGACCAGAAAAGG(+ACTCAC)GTACCAGATCTCCTGGAACTGGAATTACAAG NGG 10 8 17 8 24 0 100 5 10
    240604233426_zr7y AACCACTTGTAATTCCAGTTCCAGGAGATCTG(+ACTCAC)GTACCCTTTTCTGGTCTGGGAGAGCACCAGG NGG 10 8 17 8 24 0 100 5 10
    240604233425_49wm AGTGTGCAGACAAACGGGACATGCGATCTGAG(+ACTCAC)GATCCGAAGCCATACTGCTAACTAGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240604233423_ikgo CCCTGCAGGGTCTAGTTAGCAGTATGGCTTCG(+ACTCAC)GATCCTCAGATCGCATGTCCCGTTTGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240604233421_pa16 TCCGGCTCCTGGGTGCCACTACCAGTCCTGTG(+ACTCAC)GTACCCTGTCTATGCCTGGGACCTTCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240604233419_3b43 GCACCCAGCAGAAGGTCCCAGGCATAGACAGG(+ACTCAC)GTACCACAGGACTGGTAGTGGCACCCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240604233418_zdty GCTCTCTGGGTGGCTTACGCTGGATTGTAGTG(+ACTCAC)GATCCAGCCCCACTTGGGGACCTGCCATGTA NGG 10 8 17 8 24 0 100 5 10
    240604233416_ze6v AGAGCTACATGGCAGGTCCCCAAGTGGGGCTG(+ACTCAC)GATCCACTACAATCCAGCGTAAGCCACCCAG NGG 10 8 17 8 24 0 100 5 10