Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232421_lfhf GTCATGCCCCCTGACTTGTGGGATGCCCCACG(+ACTCAC)GATCCACTGAAGAGCTGAGGCGGTGAACAGG NGG 10 8 17 8 24 0 100 5 10
    240604232420_vin2 AACATCCTGTTCACCGCCTCAGCTCTTCAGTG(+ACTCAC)GATCCGTGGGGCATCCCACAAGTCAGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240604232418_jqxl ATGAAGGGTGGGACAGGTGCAGGCACACACAG(+ACTCAC)GTACCCAAGAACAGAGCTCTCAGCGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604232417_hqjd AGTGCCCCAGCCGCTGAGAGCTCTGTTCTTGG(+ACTCAC)GTACCTGTGTGTGCCTGCACCTGTCCCACCC NGG 10 8 17 8 24 0 100 5 10
    240604232415_iqnx TGATAAAGCCAAAAAAAAAAAAAGCAATATAG(+ACTCAC)GTACCAGTGAACTCTGGGACAAATTCATTCC NGG 10 8 17 8 24 0 100 5 10
    240604232414_7nci AATTTGGAATGAATTTGTCCCAGAGTTCACTG(+ACTCAC)GTACCTATATTGCTTTTTTTTTTTTTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240604232413_73sc TGTTTTTCTGTTTGTGTTCACTCCTTTTCTAG(+ACTCAC)GATCCAGGCAAAGGTGTAAGCCGAGCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240604232411_rig7 TTGAGTTAAAGCTCGGCTTACACCTTTGCCTG(+ACTCAC)GATCCTAGAAAAGGAGTGAACACAAACAGAA NGG 10 8 17 8 24 0 100 5 10
    240604232410_r228 TTCTCGCTCCCTGGAGAGCCTGGAAGACACTG(+ACTCAC)GATCCTCAGAGAGACAAGGCGGCGTGCTGAC NGG 10 8 17 8 24 0 100 5 10
    240604232409_lgnd AGGCTGTCAGCACGCCGCCTTGTCTCTCTGAG(+ACTCAC)GATCCAGTGTCTTCCAGGCTCTCCAGGGAGC NGG 10 8 17 8 24 0 100 5 10