Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604220824_5kbg TTGTGTGTATTTATTAGAGCATTTGCATACTG(+ACTCAC)GTACCTTTTATTAAAAGGGTTGATTTGGTGT NGG 10 8 17 8 24 0 100 5 10
    240604220823_ijd8 GTAAAACACCAAATCAACCCTTTTAATAAAAG(+ACTCAC)GTACCAGTATGCAAATGCTCTAATAAATACA NGG 10 8 17 8 24 0 100 5 10
    240604220822_3ddd TGATTCTTTAACCGTGGGACCACCTGAAAAGG(+ACTCAC)GATCCTCCTCTGCAGAGCTGTGCCTGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240604220820_eq5n ACTGCTAAGACAGGCACAGCTCTGCAGAGGAG(+ACTCAC)GATCCCTTTTCAGGTGGTCCCACGGTTAAAG NGG 10 8 17 8 24 0 100 5 10
    240604220819_x1lf TCTTGTAGCCATCACCTGCCTGGGCAGGCACG(+ACTCAC)GATCCCTCTATACCATTTACCCAGCAAACCA NGG 10 8 17 8 24 0 100 5 10
    240604220817_ipo3 ATGGCTGGTTTGCTGGGTAAATGGTATAGAGG(+ACTCAC)GATCCGTGCCTGCCCAGGCAGGTGATGGCTA NGG 10 8 17 8 24 0 100 5 10
    240604220814_9ths CATCTATCCATAGGTGACCTGCTTGGCCCATG(+ACTCAC)GATCCATTCTCATGGTCAGCACTCAACAGTG NGG 10 8 17 8 24 0 100 5 10
    240604220813_1ahq GAACCCACTGTTGAGTGCTGACCATGAGAATG(+ACTCAC)GATCCATGGGCCAAGCAGGTCACCTATGGAT NGG 10 8 17 8 24 0 100 5 10
    240604220811_e7w0 AAATAAAAAAGAGAAACTCTGGGAACCTCTGG(+ACTCAC)GATCCGAGGCAGCCAATACAGTGGTGTAAGT NGG 10 8 17 8 24 0 100 5 10
    240604220809_zdvv ATCCAACTTACACCACTGTATTGGCTGCCTCG(+ACTCAC)GATCCCAGAGGTTCCCAGAGTTTCTCTTTTT NGG 10 8 17 8 24 0 100 5 10