Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604220642_vtlj TTAAAATAGAGAAATTGACAGCATTCACCATG(+ACTCAC)GTACCCAGGGGTTAGAGCTGGTGTCAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240604220639_u5u5 CGATTACTCTTGACACCAGCTCTAACCCCTGG(+ACTCAC)GTACCATGGTGAATGCTGTCAATTTCTCTAT NGG 10 8 17 8 24 0 100 5 10
    240604220637_ppi2 CTCCTCCTCCCTCCTCCCTCAGAAGTTCTGAG(+ACTCAC)GATCCTCGACAAGGTCACTGTCCACGACACT NGG 10 8 17 8 24 0 100 5 10
    240604220636_jhai TCATTAGTGTCGTGGACAGTGACCTTGTCGAG(+ACTCAC)GATCCTCAGAACTTCTGAGGGAGGAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240604220634_3gbv GGTTGGGAATGTGGCAAATCATGGCCAGTCTG(+ACTCAC)GATCCCCTGGTCAGTGCTCTCTGTATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604220631_2gvj AGGAGCCCAGATACAGAGAGCACTGACCAGGG(+ACTCAC)GATCCAGACTGGCCATGATTTGCCACATTCC NGG 10 8 17 8 24 0 100 5 10
    240604220630_e0a4 TTAATTAGTCTAGCTACCCAGCTTGCTGTAGG(+ACTCAC)GATCCCTTTGTGCTTTTTTAAGGAGTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604220628_1u5w TGAATTTTCTACTCCTTAAAAAAGCACAAAGG(+ACTCAC)GATCCCTACAGCAAGCTGGGTAGCTAGACTA NGG 10 8 17 8 24 0 100 5 10
    240604220627_wmzh ACATCTGGGGTCAAGAGAAAGGGGAGTGAGAG(+ACTCAC)GATCCTCAAAGGATATCTCCTTTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240604220626_orxn AGGAAGGAAGGGCAAAGGAGATATCCTTTGAG(+ACTCAC)GATCCTCTCACTCCCCTTTCTCTTGACCCCA NGG 10 8 17 8 24 0 100 5 10