Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604210827_ae6v CACTTGCCGCTCTTGCAGAGAACTCTGGTTTG(+ACTCAC)GATCCTGTCATCACATGGCAGCTCAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240604210826_hn6x TCTGTACCTAGGGTCTGAACTTAGTTAATGAG(+ACTCAC)GATCCCAGCAAGACTCTCCAGTTTACATTCC NGG 10 8 17 8 24 0 100 5 10
    240604210824_9aqb GGTGAGGAATGTAAACTGGAGAGTCTTGCTGG(+ACTCAC)GATCCTCATTAACTAAGTTCAGACCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240604210823_28tr TTTTAGTACCGACATATATTATTTTCATTGTG(+ACTCAC)GATCCATTTTTTATTGCATGTTCAGATGTCA NGG 10 8 17 8 24 0 100 5 10
    240604210821_ar43 GCAAATGACATCTGAACATGCAATAAAAAATG(+ACTCAC)GATCCACAATGAAAATAATATATGTCGGTAC NGG 10 8 17 8 24 0 100 5 10
    240604210820_kiqx GCTGTTTCAAGAGCATTAGGTACATGCACATG(+ACTCAC)GTACCTTCCTAATTAATTGACCTGGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604210819_xqeg CAGCTCTTTTTCCAGGTCAATTAATTAGGAAG(+ACTCAC)GTACCATGTGCATGTACCTAATGCTCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240604210817_gq6s ATAACTGTTAACTGCAACATATCTTTGGCAGG(+ACTCAC)GATCCTCAGTCGTACATTTTGTCTTTAAATG NGG 10 8 17 8 24 0 100 5 10
    240604210816_o71s AGAGGCATTTAAAGACAAAATGTACGACTGAG(+ACTCAC)GATCCCTGCCAAAGATATGTTGCAGTTAACA NGG 10 8 17 8 24 0 100 5 10
    240604210815_37mw TCTGAGAGCAGTGTAAACCTCACAGGGCTTTG(+ACTCAC)GATCCCCTCGGACCCCCTTGGACACAGAGGA NGG 10 8 17 8 24 0 100 5 10