Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604205147_1bts ACTCCATAGATCTCAGTAATTCTGGAAACAAG(+ACTCAC)GTACCATGACATGCAGGTCATCATTGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604205145_xqaa TTTGGTTTTCCAATGATGACCTGCATGTCATG(+ACTCAC)GTACCTTGTTTCCAGAATTACTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240604205143_zt3d AAAAGGATATACCTTCTTCTCAGCACCTCATG(+ACTCAC)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240604205142_8f7r TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+ACTCAC)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240604205140_k09t ACGAAATGGACAAATTTCTGGACAGATACCAG(+ACTCAC)GTACCAAAGTTGAATCAGGATCAAGTTGACC NGG 10 8 17 8 24 0 100 5 10
    240604205138_n91u TAGAAGGTCAACTTGATCCTGATTCAACTTTG(+ACTCAC)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240604205137_grhg TATCTGAAACATTAACCAGTTCATCTTCATAG(+ACTCAC)GATCCATTTGAAGCCAAATCATGTCATGGTG NGG 10 8 17 8 24 0 100 5 10
    240604205136_ke0s GTGTGCACCATGACATGATTTGGCTTCAAATG(+ACTCAC)GATCCTATGAAGATGAACTGGTTAATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240604205134_vo0n TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ACTCAC)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240604205132_626x TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ACTCAC)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10