Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604201041_ca4r GAGGAACCTTCAACGTAGGCAGCTATTAAATG(+ACTCAC)GATCCAGTATTTCTCAGAGCATCCTATGCAA NGG 10 8 17 8 24 0 100 5 10
    240604201040_y2nb TCACCTTGCATAGGATGCTCTGAGAAATACTG(+ACTCAC)GATCCATTTAATAGCTGCCTACGTTGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240604201039_tl0b TGAGTGACCTGGGATACATAGACACCCTTATG(+ACTCAC)GATCCTTGACATCTGTGCTCCATTGCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240604201038_ssd6 GGCTTTTGGAGCAATGGAGCACAGATGTCAAG(+ACTCAC)GATCCATAAGGGTGTCTATGTATCCCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240604201036_fbht TCCCTGGCTCCACTATCGAACTGCTGGGTGGG(+ACTCAC)GATCCGTTCCTGCTATTACCCTCTTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240604201034_jvbz CATCTCAGTAGAAGAGGGTAATAGCAGGAACG(+ACTCAC)GATCCCCACCCAGCAGTTCGATAGTGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604201033_udl8 GACCCTTGGGTGTTCAGAACTCCTGCCAGGAG(+ACTCAC)GATCCTCAGTGCCCACTAAGTCCATGCCATC NGG 10 8 17 8 24 0 100 5 10
    240604201031_oqjx CTGGGGATGGCATGGACTTAGTGGGCACTGAG(+ACTCAC)GATCCTCCTGGCAGGAGTTCTGAACACCCAA NGG 10 8 17 8 24 0 100 5 10
    240604201030_cosu TTCAATAAATACTTGTTGCATGAACAAACTTG(+ACTCAC)GATCCAAATTTTTAGAAATATTGAATTTGGA NGG 10 8 17 8 24 0 100 5 10
    240604201029_i9w0 GAGCATCCAAATTCAATATTTCTAAAAATTTG(+ACTCAC)GATCCAAGTTTGTTCATGCAACAAGTATTTA NGG 10 8 17 8 24 0 100 5 10