Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613154352_0yqc CACCAGGCTAGGCTCATGGCCTTGAACTCAGG(+AGGACA)GATCCACCTGCCTCTGCCTCCCTGTGATGCT NGG 10 8 17 8 24 0 100 5 10
    240613154350_l95d ATCTCAGCATCACAGGGAGGCAGAGGCAGGTG(+AGGACA)GATCCCTGAGTTCAAGGCCATGAGCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240613154349_0enl GCCTGAAGAGGGCCTGGGGAACAGTGGAAATG(+AGGACA)GTACCTTCATGGTGACTTCAGCAGGGTAGTC NGG 10 8 17 8 24 0 100 5 10
    240613154348_gij5 GTAATGACTACCCTGCTGAAGTCACCATGAAG(+AGGACA)GTACCATTTCCACTGTTCCCCAGGCCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613154346_bfaw ATAGCTGTGGTATCAGGACTTGAGAGATGGAG(+AGGACA)GATCCAAAAGTTTAGGGTCAGTCTGGGTATA NGG 10 8 17 8 24 0 100 5 10
    240613154345_bln4 TGCTGTATACCCAGACTGACCCTAAACTTTTG(+AGGACA)GATCCTCCATCTCTCAAGTCCTGATACCACA NGG 10 8 17 8 24 0 100 5 10
    240613154342_vo4b TCCTGCCAGCCCTTGAGCCTCTGCTCTCCTGG(+AGGACA)GTACCCTCCTGGAACACCATGCTAACCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613154340_d7ty CTCGGGGAAGGTTAGCATGGTGTTCCAGGAGG(+AGGACA)GTACCCAGGAGAGCAGAGGCTCAAGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613154339_8g0g GTGTCCTGTTTGATGTCTGAGGTCTGCTTTGG(+AGGACA)GTACCGCCCTGGGAACTGCTAACCTCCGATT NGG 10 8 17 8 24 0 100 5 10
    240613154337_k5ca GGACCAATCGGAGGTTAGCAGTTCCCAGGGCG(+AGGACA)GTACCCAAAGCAGACCTCAGACATCAAACAG NGG 10 8 17 8 24 0 100 5 10