Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231107172918_vuzz CTGAGTAGTAACGCTCCTTGAGGTTGTGCAGCACCG(/A)AGGCTTCGTTGAGGCACGTGAGCTCTGCCATGTCC NGG 10 8 17 8 24 0 100 5 10
    231107172714_cjjn AGGTGTGGTAGCGCGTGGCCGAGCCCATGGCCGACATGCCGATGCCGATGCCCGAGGTGACCGGCGTCGGGGAGTAGGTGAAGGC(-G)CCTGGATAGTGCATGCGGGGGTCGGAGATGGAGGGCAGCGCGGGGAACTGGCGCGGGTCGCTGAACGCTGTCAGGTCGGGTGCC NGG 10 8 17 8 24 0 100 5 10
    231107172518_lyhn CCCGAGCAGCGGCCCCTCCGGTCCCCCCTGGACAACATGATCCGCCGCCTCTCCCC(/T)GGCTGAGAGAGCTCAGGGACCCAGGCCGCACCAGGAGAACAACCACCAGGAGTCCTAC NGG 10 8 17 8 24 0 100 5 10
    231107172123_ksax TGGTAACCCGGAGGGAGAGATTGGAAACC(/A)GCGGAGTTTCCTTTGGGAGGCTGCGGCC NGG 10 8 17 8 24 0 100 5 10
    231107172121_h7gr TGGTAACCCGGAGGGAGAGATTGGAAACC(/A)GCGGAGTTTCCTTTGGGAGGCTGCGGCC NGG 10 8 17 8 24 0 100 5 10
    231107161431_32i4 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231107154145_7wlc GAGCCTGCCCGCGGCGGTCGCCGGGCCGCGGCTTCGCCTAGGCTCG(/A)CAGAGCGGAGCGCGTGGGGCGCGGCGGCGGCG NGG 10 8 17 8 24 0 100 5 10
    231107153443_eocy CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231107153316_uue5 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231107153219_7ml1 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10