Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    231102001838_l6dl cacaaaatcaaagaagtcttataacctaggaagtcctttcttcactttcttctcccaaacacctacaaaatttgcttcctcctttgtgtctttacccaaatttaatctctatatgtctttctctaaccaacttatttctactcatccctgccaccagtgcgtctgtaatacttattaacttgtaatatattacataaggtaagctccatgagggtaga(/t)aattttgttctttctattttctgtttttcctccagcccctggaacaatacctgacacagagtaagcactcaataaataattggtgaaggcattaatcccagaggcagttcagcaagaggctgatggagtggttctgggactc NGG 10 8 17 8 24 0 100 5 10
    231102000437_jjuf aatacttattaacttgtaatatattacataaggtaagctccatgagggtagA(/T)aattttgttctttctattttctgtttttcctccagcccctggaacaatacctgacaca NGG 10 8 17 8 24 0 100 5 10
    231101171917_tla2 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231031185209_g20l GGAAAAAAATGAAGCAATAGCAGTTGCCGAGGTCGGCTGTAAAACAAACAAACAAAAACAAACTCAGGAGTA(-G)CAAGGAGGAGGGGAGAGGAGGCATCTGTATTGGCGGATCTCGGGAGAGAAGGATCACTCCGCGAATGGGAGGCAGTCCACTCTAGAAAGCCAGACCAT NGG 10 8 17 8 24 0 100 5 10
    231031184447_jnaa CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231031184213_lnwg CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231031171811_wdmg CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231031151429_65j5 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    231030210429_69qe GCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAAGATGC(/G)TGTGGCCGATGTGTCTATTGAAGATTCTGTGATCTCACTCTCAGGAGACCATTGCATCATTGGCCGCACACTGGTG NGG 10 8 17 8 24 0 100 5 10
    231030203918_tcla GCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAAGATGG(/C)TGTGGCCGATGTGTCTATTGAAGATTCTGTGATCTCACTCTCAGGAGACCATTGCATCATTGGCCGCACACTGGTG NGG 10 8 17 8 24 0 100 5 10