Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152708_uo9q AAATACAGACTTCGCTCTCTAGGCTTTTGCTG(+AGGACA)GTACCAGGAACCCAATATGGCCCTTGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613152706_8wbv CCTGTAGCCTCAAGGGCCATATTGGGTTCCTG(+AGGACA)GTACCAGCAAAAGCCTAGAGAGCGAAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613152705_b6gm CCCAGCCAGTGGAAGCTGCAAGAAGACATATG(+AGGACA)GTACCACTGGGCTGCTGCTCTCCCAGACTTA NGG 10 8 17 8 24 0 100 5 10
    240613152703_oll2 AGCTCTAAGTCTGGGAGAGCAGCAGCCCAGTG(+AGGACA)GTACCATATGTCTTCTTGCAGCTTCCACTGG NGG 10 8 17 8 24 0 100 5 10
    240613152700_hqo0 CCATAGATCAAGTGCACATATCCCTGACCCAG(+AGGACA)GTACCATGGTTCATGGTTCCCCAGCTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613152659_p7lr CATCTAAACCAGCTGGGGAACCATGAACCATG(+AGGACA)GTACCTGGGTCAGGGATATGTGCACTTGATC NGG 10 8 17 8 24 0 100 5 10
    240613152657_1ldd CACTCCTATGATGATGATACAAAGCCTCTAAG(+AGGACA)GATCCCCAAGCCCCAGGTATAACTGGCATCC NGG 10 8 17 8 24 0 100 5 10
    240613152655_i11c ATGTGGGATGCCAGTTATACCTGGGGCTTGGG(+AGGACA)GATCCTTAGAGGCTTTGTATCATCATCATAG NGG 10 8 17 8 24 0 100 5 10
    240613152654_k6bi CTGTGCTTCTAATCATCCAAAATAACTCCTTG(+AGGACA)GTACCACAGGAAGCTGGGTAGCTCCCTCATG NGG 10 8 17 8 24 0 100 5 10
    240613152652_1nfg CCTACCATGAGGGAGCTACCCAGCTTCCTGTG(+AGGACA)GTACCAAGGAGTTATTTTGGATGATTAGAAG NGG 10 8 17 8 24 0 100 5 10