Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152619_jpvk TGGTTGCTTATTTCTCCCATGGAGAAACAAAG(+AGGACA)GATCCTACTGGAACATTGTCCTCACACCTGA NGG 10 8 17 8 24 0 100 5 10
    240613152617_aal6 GACCATCAGGTGTGAGGACAATGTTCCAGTAG(+AGGACA)GATCCTTTGTTTCTCCATGGGAGAAATAAGC NGG 10 8 17 8 24 0 100 5 10
    240613152616_o8mg AGATCTTAACTTACAAATAATTTATAGGGATG(+AGGACA)GATCCACTCCCTCATAATTTGATCTGCAACA NGG 10 8 17 8 24 0 100 5 10
    240613152615_nth1 TGATCTGTTGCAGATCAAATTATGAGGGAGTG(+AGGACA)GATCCATCCCTATAAATTATTTGTAAGTTAA NGG 10 8 17 8 24 0 100 5 10
    240613152613_fcja AATTGCTTAGGTTAGTTCATCTGGAACACAGG(+AGGACA)GTACCACTATGAAGCTCAATTTCACTTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613152612_4y3o TATCTGCTCAAGTGAAATTGAGCTTCATAGTG(+AGGACA)GTACCCTGTGTTCCAGATGAACTAACCTAAG NGG 10 8 17 8 24 0 100 5 10
    240613152611_ps6v AACATTTATACAAACCCTGTAACTCCATCAAG(+AGGACA)GATCCATTTGAATGGTATTGAAGTCAGCCAA NGG 10 8 17 8 24 0 100 5 10
    240613152610_gr3j GTAGCTTGGCTGACTTCAATACCATTCAAATG(+AGGACA)GATCCTTGATGGAGTTACAGGGTTTGTATAA NGG 10 8 17 8 24 0 100 5 10
    240613152608_a23h TTAGAGAAGGTGGTCCCTCAGGATGCAGCTGG(+AGGACA)GATCCACGCAGGCCCCAGGACACTAGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613152607_32ih GTAGGGCAGCCTAGTGTCCTGGGGCCTGCGTG(+AGGACA)GATCCCAGCTGCATCCTGAGGGACCACCTTC NGG 10 8 17 8 24 0 100 5 10