Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152550_9ocm TACCGCTTTCTCTGAGCACTCTGGGTGGCCAG(+AGGACA)GTACCATCAACGTTTTGATCCAATACCTATG NGG 10 8 17 8 24 0 100 5 10
    240613152548_qvrb GCTGTCATAGGTATTGGATCAAAACGTTGATG(+AGGACA)GTACCTGGCCACCCAGAGTGCTCAGAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613152547_tw7m AACTTGGCAATAGCCAAGTCTAGAAGATGCTG(+AGGACA)GTACCAAATAGCCTAATTCTTGTGATTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613152546_0r8k GGACTCTCCAATCACAAGAATTAGGCTATTTG(+AGGACA)GTACCAGCATCTTCTAGACTTGGCTATTGCC NGG 10 8 17 8 24 0 100 5 10
    240613152545_xu0t TGGAACAAAGTCATAACCTTGTCATTGAAGAG(+AGGACA)GTACCAGAACTGAATTGAGACAAGAGAAATA NGG 10 8 17 8 24 0 100 5 10
    240613152544_25pf CCAAATATTTCTCTTGTCTCAATTCAGTTCTG(+AGGACA)GTACCTCTTCAATGACAAGGTTATGACTTTG NGG 10 8 17 8 24 0 100 5 10
    240613152542_s0ez GGGAGCAGAAAAAAATGGTCAGGGCTCTGATG(+AGGACA)GATCCAAACTTGGCTGTGCCCACTGTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613152541_hsy0 TGATGAGACAACAGTGGGCACAGCCAAGTTTG(+AGGACA)GATCCATCAGAGCCCTGACCATTTTTTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613152540_kh72 CTTTTCCTGCTTTAAAATTCAGACTGTAACAG(+AGGACA)GATCCCTTTCTAAAGGAATCTGTACATTCAT NGG 10 8 17 8 24 0 100 5 10
    240613152538_cm4w AAAGCATGAATGTACAGATTCCTTTAGAAAGG(+AGGACA)GATCCTGTTACAGTCTGAATTTTAAAGCAGG NGG 10 8 17 8 24 0 100 5 10