Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152449_xc7r GAAAGAATTGGCATGTTTTTTAATTTGCAGGG(+AGGACA)GATCCTTGTGAGCATCTAGATAGTCCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613152448_7g1z AAGAGAGAAGGGACTATCTAGATGCTCACAAG(+AGGACA)GATCCCCTGCAAATTAAAAAACATGCCAATT NGG 10 8 17 8 24 0 100 5 10
    240613152446_w94i CCAGGGCTTTAGGATTGCTGAGTGCTCCGGAG(+AGGACA)GATCCTCACATACCCACTGTTACTGTTCACT NGG 10 8 17 8 24 0 100 5 10
    240613152444_r3ds TCTAAAGTGAACAGTAACAGTGGGTATGTGAG(+AGGACA)GATCCTCCGGAGCACTCAGCAATCCTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240613152443_b9ur TCCAGGGTATCTAACACTTCTGGCCTCCACAG(+AGGACA)GTACCTGTCTCACAGGACCATAACCCCACAG NGG 10 8 17 8 24 0 100 5 10
    240613152441_xhbt TTTCTCTGTGGGGTTATGGTCCTGTGAGACAG(+AGGACA)GTACCTGTGGAGGCCAGAAGTGTTAGATACC NGG 10 8 17 8 24 0 100 5 10
    240613152440_5moz TTGTGACAAATATTTTTGCCTTTCAAAGTTAG(+AGGACA)GTACCAGAAATATCAAGACTTGGGTTAACCA NGG 10 8 17 8 24 0 100 5 10
    240613152439_a6u4 TATCATGGTTAACCCAAGTCTTGATATTTCTG(+AGGACA)GTACCTAACTTTGAAAGGCAAAAATATTTGT NGG 10 8 17 8 24 0 100 5 10
    240613152437_01qm AATCCACAGAGGATTTCACATGATGTCAGCCG(+AGGACA)GTACCGGGAGGAGCCATGCAGGATGGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240613152435_jlaw GACAAGTGCACCATCCTGCATGGCTCCTCCCG(+AGGACA)GTACCGGCTGACATCATGTGAAATCCTCTGT NGG 10 8 17 8 24 0 100 5 10