Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152349_i44d CCGACTTGCCAGCAAGAACGACGCTGCAACAG(+AGGACA)GATCCTTCCGCACACATTTACTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613152347_g9ek AGTTAAGCTCTCCCAGTAAATGTGTGCGGAAG(+AGGACA)GATCCTGTTGCAGCGTCGTTCTTGCTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240613152346_lfr8 CTAACTCCGCTGTGATCCAGAATGGGCAACTG(+AGGACA)GTACCGCTCAGTAGTACATAAACCCCTTCAA NGG 10 8 17 8 24 0 100 5 10
    240613152345_9nvd GTTTGTTGAAGGGGTTTATGTACTACTGAGCG(+AGGACA)GTACCAGTTGCCCATTCTGGATCACAGCGGA NGG 10 8 17 8 24 0 100 5 10
    240613152343_7x8h GAGGAGACAGAAAAGGCAGTGGTGTCCACTAG(+AGGACA)GATCCTCTGTCCACCACTTGAGATAAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240613152342_hq5l ACACCGTACCTTATCTCAAGTGGTGGACAGAG(+AGGACA)GATCCTAGTGGACACCACTGCCTTTTCTGTC NGG 10 8 17 8 24 0 100 5 10
    240613152340_65ja CATTTGCAGAGTGGACCCAGCAGTGCCCACAG(+AGGACA)GATCCTTATTCACGTATCTAAGTCTGATATT NGG 10 8 17 8 24 0 100 5 10
    240613152339_9bau CATAGAATATCAGACTTAGATACGTGAATAAG(+AGGACA)GATCCTGTGGGCACTGCTGGGTCCACTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613152336_iwq2 AGTAGGACCATCCAGAAACTTCTTCACCCAGG(+AGGACA)GATCCATCCCATAGTCAGCCACCAAATGCAG NGG 10 8 17 8 24 0 100 5 10
    240613152335_s7ax AGTGTCTGCATTTGGTGGCTGACTATGGGATG(+AGGACA)GATCCCTGGGTGAAGAAGTTTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10