Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152334_36ov CAGTGAAGTTCAGTCAAGTCAAAGGTCAATTG(+AGGACA)GATCCCAATGTGGCTGCCTGAAAGGTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240613152332_pyzd TGTGGTAACAACCTTTCAGGCAGCCACATTGG(+AGGACA)GATCCAATTGACCTTTGACTTGACTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240613152331_cd0u TATCTGTTTAGTCAAAACTCTGAGTGTGCTTG(+AGGACA)GATCCCTCTGTTTGATCTGTCAAACTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613152329_baae ATGTGGGAAGAGTTTGACAGATCAAACAGAGG(+AGGACA)GATCCAAGCACACTCAGAGTTTTGACTAAAC NGG 10 8 17 8 24 0 100 5 10
    240613152328_acjj GCGCACTCTCACCAACCCATGCTCCTCTGCAG(+AGGACA)GTACCTTTCCCCTAGAGCTCAGTTGAGTCAC NGG 10 8 17 8 24 0 100 5 10
    240613152327_sxbs TTGCTGTGACTCAACTGAGCTCTAGGGGAAAG(+AGGACA)GTACCTGCAGAGGAGCATGGGTTGGTGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613152326_9fvn ACAAAAAACCCTGCCATTTCCTGTAGTTAGAG(+AGGACA)GTACCTGCAGCTTACATCTTTCTGCTGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613152325_u1ja TTTAACCTGCAGCAGAAAGATGTAAGCTGCAG(+AGGACA)GTACCTCTAACTACAGGAAATGGCAGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613152323_xttb AACAATGTCCCTGACTCTGTTGCCTGCCTGAG(+AGGACA)GATCCCATTCCCCTAACTGGACTGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613152321_edbn GACCAGACAAGGCAGTCCAGTTAGGGGAATGG(+AGGACA)GATCCTCAGGCAGGCAACAGAGTCAGGGACA NGG 10 8 17 8 24 0 100 5 10