Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152253_j8it ACACAATCAAGATTGGTCACTGTACAACAAAG(+AGGACA)GTACCTTTATGTGGCAGGGGCTAGGGACTGA NGG 10 8 17 8 24 0 100 5 10
    240613152252_3mwo TGGGTTCAGTCCCTAGCCCCTGCCACATAAAG(+AGGACA)GTACCTTTGTTGTACAGTGACCAATCTTGAT NGG 10 8 17 8 24 0 100 5 10
    240613152250_wsw6 TCCTAAAAGATGTTCCTAAGATGTTTGTTAGG(+AGGACA)GATCCACAATTCTTCCTTAGAACCTAAACAT NGG 10 8 17 8 24 0 100 5 10
    240613152249_7dml ATATAATGTTTAGGTTCTAAGGAAGAATTGTG(+AGGACA)GATCCCTAACAAACATCTTAGGAACATCTTT NGG 10 8 17 8 24 0 100 5 10
    240613152248_24g1 CAAATAAATTCTTAAACTTACCCACCAGTCTG(+AGGACA)GATCCTTGGTAGTCATTGCACATTACAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613152246_eljw TTTGCGTGCTGTAATGTGCAATGACTACCAAG(+AGGACA)GATCCAGACTGGTGGGTAAGTTTAAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240613152244_7v2w CCTTAATTTAAACATAGATACATGTCAGCCAG(+AGGACA)GTACCATCCATGATGCAGGTGTTGATAATTA NGG 10 8 17 8 24 0 100 5 10
    240613152242_wlq7 TAAGTTAATTATCAACACCTGCATCATGGATG(+AGGACA)GTACCTGGCTGACATGTATCTATGTTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240613152241_z2ke CATGTTAATGTGGGTCTGCATCTCACCTGCTG(+AGGACA)GATCCTGGGGTCCTGCACCTCACCTCTACAG NGG 10 8 17 8 24 0 100 5 10
    240613152238_u8rl GTGAACTGTAGAGGTGAGGTGCAGGACCCCAG(+AGGACA)GATCCAGCAGGTGAGATGCAGACCCACATTA NGG 10 8 17 8 24 0 100 5 10