Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152220_6yfb ACATGAGCTCACCAGGAGCTGCTGTTTTCTGG(+AGGACA)GTACCTTCTGTAGAGAACTTACCCATCACAT NGG 10 8 17 8 24 0 100 5 10
    240613152219_3jur CACCCATGTGATGGGTAAGTTCTCTACAGAAG(+AGGACA)GTACCCAGAAAACAGCAGCTCCTGGTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613152217_54qf CCTCTAAAGGAGGATTAAGAAGGTAAGAGTTG(+AGGACA)GTACCTAACCTAGATATGGAAGACTTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613152216_9rp0 TATTGTGACTAAGTCTTCCATATCTAGGTTAG(+AGGACA)GTACCAACTCTTACCTTCTTAATCCTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240613152214_3nid TTCATCTCTCTGAACACGTTTATCCCAACCTG(+AGGACA)GATCCAGGCTGCAGTAAGTGCTCAGTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240613152212_uo8s GCTCAACCCCACTGAGCACTTACTGCAGCCTG(+AGGACA)GATCCAGGTTGGGATAAACGTGTTCAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613152211_j0xr ACAGGTAAAGGAATTAGGCTTGGGGGACTTGG(+AGGACA)GATCCTAGGGAAGAAAGCTCGGGCGATCGGC NGG 10 8 17 8 24 0 100 5 10
    240613152208_t6dn ACTCAGCCGATCGCCCGAGCTTTCTTCCCTAG(+AGGACA)GATCCCAAGTCCCCCAAGCCTAATTCCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613152206_mpjp CTGGTGACAAATGGCAGATGTGCCATATATGG(+AGGACA)GATCCTTTACTTGGAAAGAAATTAGTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613152205_nrf7 CCTTCCCCTGACTAATTTCTTTCCAAGTAAAG(+AGGACA)GATCCCATATATGGCACATCTGCCATTTGTC NGG 10 8 17 8 24 0 100 5 10