Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152136_pi1z GCTGCAGTGTCTTACTTCAGCAGTCAGGCTGG(+AGGACA)GTACCCAGCAGCTGGCTTGGGTGGTGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240613152134_pzf7 TAGAACAGGAAACGAGTAACATCTGGCCAAAG(+AGGACA)GATCCCAACAGAAGGAACCTGATAAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240613152133_97zi CTTTTTCATTTTTATCAGGTTCCTTCTGTTGG(+AGGACA)GATCCTTTGGCCAGATGTTACTCGTTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613152131_0ggf GAGCAGGGGCAAGCTCTCGGGCTGAAGGGTGG(+AGGACA)GATCCACGCGTGGCTCGAGGACACAAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613152130_izk3 CCGGTTTCCCTTGTGTCCTCGAGCCACGCGTG(+AGGACA)GATCCCACCCTTCAGCCCGAGAGCTTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240613152128_8ypd AAGGAAATGGTGACCAGGATGTCGGGGTGGGG(+AGGACA)GATCCACTTCTGCTTCTTGAGGGCTGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613152126_2wfy CTCCCCAAGACAGCCCTCAAGAAGCAGAAGTG(+AGGACA)GATCCCCCACCCCGACATCCTGGTCACCATT NGG 10 8 17 8 24 0 100 5 10
    240613152124_nsnd CGAAGAGGACCCAGACCAAGAAGGCCAACCTG(+AGGACA)GATCCATGAAATTCAGAGGCAATGAGGTTAA NGG 10 8 17 8 24 0 100 5 10
    240613152123_7qfe TTAATTTAACCTCATTGCCTCTGAATTTCATG(+AGGACA)GATCCAGGTTGGCCTTCTTGGTCTGGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240613152122_t4r6 CTAAACAAAGTGATGTGTATGAAGATAGCTGG(+AGGACA)GATCCTTAGAATTCCTTCTTTTTTTCTTTCA NGG 10 8 17 8 24 0 100 5 10