Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152121_6v53 ATTTTTGAAAGAAAAAAAGAAGGAATTCTAAG(+AGGACA)GATCCCAGCTATCTTCATACACATCACTTTG NGG 10 8 17 8 24 0 100 5 10
    240613152119_0niw CATGCATTCCAAGGAAAAAGGATGGGTCAGAG(+AGGACA)GATCCAATTCACTTTTAGGCTTCCGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240613152118_5nh5 GTAAAACTGTCCGGAAGCCTAAAAGTGAATTG(+AGGACA)GATCCTCTGACCCATCCTTTTTCCTTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240613152117_58md TCATACATGCATACGAATAAATATATTTATAG(+AGGACA)GATCCATTCCTTTAAAATACGAATAAATTTA NGG 10 8 17 8 24 0 100 5 10
    240613152116_xxj7 TCCTATAAATTTATTCGTATTTTAAAGGAATG(+AGGACA)GATCCTATAAATATATTTATTCGTATGCATG NGG 10 8 17 8 24 0 100 5 10
    240613152115_d222 ACCCAGATGAAGGCTGCTTTTCTACATCATAG(+AGGACA)GTACCCTTAGGGGCCAATAACTATATATTAG NGG 10 8 17 8 24 0 100 5 10
    240613152113_3lo9 CTATTCTAATATATAGTTATTGGCCCCTAAGG(+AGGACA)GTACCTATGATGTAGAAAAGCAGCCTTCATC NGG 10 8 17 8 24 0 100 5 10
    240613152111_7wrl TTCAATTCCCAGTACCCATGTCAGCTCTAGGG(+AGGACA)GATCCAATGTTCTCTCCTGTCCTCTAGAACC NGG 10 8 17 8 24 0 100 5 10
    240613152110_q2ri GCACTGGTTCTAGAGGACAGGAGAGAACATTG(+AGGACA)GATCCCCTAGAGCTGACATGGGTACTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240613152108_isze GTGTCTACTTCGTAAGAGCCGTGTCTATCCAG(+AGGACA)GTACCAAAAGTGAGAGGTCAGCAGTGGGTGG NGG 10 8 17 8 24 0 100 5 10