Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152523_n4hd AGCGTTTGCCAAGATGGAGCTTTATACAGATG(+AGGACA)GTACCTCCTAAGTCCCCTCTCTGGCCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240613152521_umzk ATGCAATGCTGGCCAGAGAGGGGACTTAGGAG(+AGGACA)GTACCATCTGTATAAAGCTCCATCTTGGCAA NGG 10 8 17 8 24 0 100 5 10
    240613152520_zaky GCATATATGTATGTAAGCAGACCACGTGGGTG(+AGGACA)GTACCTGTAGGGTATCAGAAGAGGGCATCAG NGG 10 8 17 8 24 0 100 5 10
    240613152519_2vf8 GGGATCTGATGCCCTCTTCTGATACCCTACAG(+AGGACA)GTACCACCCACGTGGTCTGCTTACATACATA NGG 10 8 17 8 24 0 100 5 10
    240613152517_msz3 ACCCCCAGGAGAGAGTGGTGGAGAGAGTGGAG(+AGGACA)GATCCCGCAGGTCTTTGCCAATTAAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240613152516_4u2s TACCCCAAAGTTTAATTGGCAAAGACCTGCGG(+AGGACA)GATCCTCCACTCTCTCCACCACTCTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613152512_dy3p AGAAAACAGCCTGGAAGGGTAAGTACCCCCGG(+AGGACA)GTACCATACACTTCTGAAATCCCGAGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240613152511_8de6 GCCCCAAAGGCTCGGGATTTCAGAAGTGTATG(+AGGACA)GTACCCGGGGGTACTTACCCTTCCAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613152509_n95d CTCAGCCTTCCACGTGCTGTGATTATAGGCAG(+AGGACA)GTACCACCATATACAGCATATTGTATGTGTC NGG 10 8 17 8 24 0 100 5 10
    240613152508_0i65 TTATAGACACATACAATATGCTGTATATGGTG(+AGGACA)GTACCTGCCTATAATCACAGCACGTGGAAGG NGG 10 8 17 8 24 0 100 5 10