Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152537_5loq CTCTTTGTAGCTGAGGGTGACTTTAAACTTCG(+AGGACA)GATCCTCCTGCTTACCCACCCCCAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613152535_pun4 AATCCCAGCACTTGGGGGTGGGTAAGCAGGAG(+AGGACA)GATCCGAAGTTTAAAGTCACCCTCAGCTACA NGG 10 8 17 8 24 0 100 5 10
    240613152534_9b4h TGGGTAGAGATTTGGTCCAGCGCATTCTCATG(+AGGACA)GATCCTTCTCATCTTTTCCCAGGAACACTCG NGG 10 8 17 8 24 0 100 5 10
    240613152533_3pmo GGGAGCGAGTGTTCCTGGGAAAAGATGAGAAG(+AGGACA)GATCCATGAGAATGCGCTGGACCAAATCTCT NGG 10 8 17 8 24 0 100 5 10
    240613152532_6zd0 GCTGTTTTCAAACACACCAGAAGAGGGCATAG(+AGGACA)GATCCCATTACAGAGGGTTGTGAGTCACGGT NGG 10 8 17 8 24 0 100 5 10
    240613152530_g7p3 ACCACACCGTGACTCACAACCCTCTGTAATGG(+AGGACA)GATCCTATGCCCTCTTCTGGTGTGTTTGAAA NGG 10 8 17 8 24 0 100 5 10
    240613152529_vz1w AAAGCAGTGGTTTGTACACACAGTACTGAAAG(+AGGACA)GTACCCGAGGAGTCGCTACTCACAGTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240613152527_buf6 ACATATTTAAACTGTGAGTAGCGACTCCTCGG(+AGGACA)GTACCTTTCAGTACTGTGTGTACAAACCACT NGG 10 8 17 8 24 0 100 5 10
    240613152525_znfs CTGACAGACAGCCAAAATGGGGCAGGTTCCAG(+AGGACA)GATCCTGATTGGTCGAGTGCTGGAGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613152524_sf9b GGCTCTGCCTCCTCCAGCACTCGACCAATCAG(+AGGACA)GATCCTGGAACCTGCCCCATTTTGGCTGTCT NGG 10 8 17 8 24 0 100 5 10