Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152738_bp1d CACATGGTGGCTCACAACCATCTGTAATGGGG(+AGGACA)GATCCGATGCCTTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613152736_wldl GTCAGGGTGAAGGAGGTCACCTAAGGACCTTG(+AGGACA)GATCCCAGTTATCACATGCAGGTGGCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613152734_th2u AGAAGACAAAGCCACCTGCATGTGATAACTGG(+AGGACA)GATCCAAGGTCCTTAGGTGACCTCCTTCACC NGG 10 8 17 8 24 0 100 5 10
    240613152732_w4eg AGACCTTAAGGGTCCAGGCCCACCCCTGCCAG(+AGGACA)GATCCCTGGTCTGCTTTCTCCAGGACACACT NGG 10 8 17 8 24 0 100 5 10
    240613152730_55f2 GTCCCAGTGTGTCCTGGAGAAAGCAGACCAGG(+AGGACA)GATCCTGGCAGGGGTGGGCCTGGACCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613152728_p3hi TCACTCATCAGGAGCAGAATGAGGGACAAAAG(+AGGACA)GTACCCAGGAATCGGGACCCAGGAATTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613152726_7tpk GTTAGAGACAATTCCTGGGTCCCGATTCCTGG(+AGGACA)GTACCTTTTGTCCCTCATTCTGCTCCTGATG NGG 10 8 17 8 24 0 100 5 10
    240613152723_qt0n AGTCTTTTTTACACTCTGCCTGTGGTCCCAGG(+AGGACA)GATCCTGGCTCAGAGGACTCAAGCGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613152721_0evd CCTGTCCCCTGCGCTTGAGTCCTCTGAGCCAG(+AGGACA)GATCCCTGGGACCACAGGCAGAGTGTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613152720_krd5 CTTGTGGGGCTTTTAGTTTGAAGACCCTGAAG(+AGGACA)GATCCCACTGCATAGTGAGTCTGTCCTGGTT NGG 10 8 17 8 24 0 100 5 10