Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151849_ykz7 GCAGAGCAGTAAGCAAAGGGGCCAGCTGTACG(+AGGACA)GTACCTGGAGAAAGACCTGGGCCTTCCATTC NGG 10 8 17 8 24 0 100 5 10
    240613151848_1ov2 TCAATGGTGCTTTAAGGACGTGTGCAGGATAG(+AGGACA)GTACCTTAGAGGAGCAGCCTAGTTTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613151847_vgu0 CATCAGCAGAGAAACTAGGCTGCTCCTCTAAG(+AGGACA)GTACCTATCCTGCACACGTCCTTAAAGCACC NGG 10 8 17 8 24 0 100 5 10
    240613151846_f92k GTCCTGTTAGATGGAATCCTGTCATCTCATTG(+AGGACA)GTACCCAGGGAGCCCAACTTCTTAGTAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613151843_o37p CAAGAGAGCTACTAAGAAGTTGGGCTCCCTGG(+AGGACA)GTACCAATGAGATGACAGGATTCCATCTAAC NGG 10 8 17 8 24 0 100 5 10
    240613151842_906p TGGGTAACTACGGAGGCCAAAAGAAGGCATTG(+AGGACA)GATCCCCTGGAGCTGTAGTTACAGGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613151839_inrl GCTCACAAGCACCTGTAACTACAGCTCCAGGG(+AGGACA)GATCCAATGCCTTCTTTTGGCCTCCGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613151837_jf18 GGCAGGTGAGTAGCTCTGAGCTCCTGCCTCAG(+AGGACA)GTACCTTAACATGGACAGCAATGTGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240613151836_4z04 ATTCCAGTTTCCACATTGCTGTCCATGTTAAG(+AGGACA)GTACCTGAGGCAGGAGCTCAGAGCTACTCAC NGG 10 8 17 8 24 0 100 5 10
    240613151835_25u8 AGGTACTTCCTAAAGCTGCCCTGCCTTTACAG(+AGGACA)GATCCCCCGAAAGAGAATTTAGGACGAGGCA NGG 10 8 17 8 24 0 100 5 10