Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613152033_codn AGGACACAGTGACGGATTAGCTCCGTTCTGCG(+AGGACA)GTACCTTCCTCTTCCCCATTAACTTGTGCCA NGG 10 8 17 8 24 0 100 5 10
    240613152031_fc9s GTGTGCTGGTCCAGCAGACAGCAGGACCAGAG(+AGGACA)GTACCCGGCGACTCTTGGCAAACACCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613152029_zp0w GCCCAAGAAAGGTGTTTGCCAAGAGTCGCCGG(+AGGACA)GTACCTCTGGTCCTGCTGTCTGCTGGACCAG NGG 10 8 17 8 24 0 100 5 10
    240613152027_5xft AGCAGAGTTTTAAAACCATCTCAGCCACCTCG(+AGGACA)GATCCCAGAGAAGGACGACACTGAGTTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613152025_ho8d GGGGCTCAAAACTCAGTGTCGTCCTTCTCTGG(+AGGACA)GATCCGAGGTGGCTGAGATGGTTTTAAAACT NGG 10 8 17 8 24 0 100 5 10
    240613152024_d3ly CTGAAGTCTAGAAGCGTTGTCTCTCATCTGTG(+AGGACA)GTACCCTCCCTGCGGTTCTCCAGTACCACTC NGG 10 8 17 8 24 0 100 5 10
    240613152022_rflc AAACTGAGTGGTACTGGAGAACCGCAGGGAGG(+AGGACA)GTACCACAGATGAGAGACAACGCTTCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240613152020_o11j TCACAGATAGGTCCAAATAGCACTATCCCAAG(+AGGACA)GATCCCACAAAAGAACTTGGGCATACTTATT NGG 10 8 17 8 24 0 100 5 10
    240613152018_agky GTATCAATAAGTATGCCCAAGTTCTTTTGTGG(+AGGACA)GATCCTTGGGATAGTGCTATTTGGACCTATC NGG 10 8 17 8 24 0 100 5 10
    240613152016_yzwq CAGCAGGCCTGGTAGCCCAGTGATGAGAAAGG(+AGGACA)GATCCGCCCTTCCCTGTGGATGTATGAGCAG NGG 10 8 17 8 24 0 100 5 10