Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613151727_2hav ACATTGACTCTAAGTGGATGGGAGTGCTGGAG(+AGGACA)GATCCTATGCAGGCTGGAGGACCCTGCGCTT NGG 10 8 17 8 24 0 100 5 10
    240613151725_tgcr AGCTTCTACCCATTCTCTCTCCCACTTCTGAG(+AGGACA)GATCCCCACAGCTAACATGCAATGCCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613151723_kjmm GTAGGGAGCAGGCATTGCATGTTAGCTGTGGG(+AGGACA)GATCCTCAGAAGTGGGAGAGAGAATGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240613151722_0532 TTTACACTGTAGTATCTCTGTGATCACTGCTG(+AGGACA)GATCCTCTGTATTCCGAAACAGGTACAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613151721_m5zi TGTCTGTTTTGTACCTGTTTCGGAATACAGAG(+AGGACA)GATCCAGCAGTGATCACAGAGATACTACAGT NGG 10 8 17 8 24 0 100 5 10
    240613151720_33gk GCCTGAGATTCCATTTCTTCTTCTCTTCACAG(+AGGACA)GTACCTGAATTCAGGTGTGTCTCTTGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240613151719_rq9b AATCTCAGGGCAAGAGACACACCTGAATTCAG(+AGGACA)GTACCTGTGAAGAGAAGAAGAAATGGAATCT NGG 10 8 17 8 24 0 100 5 10
    240613151717_r96z GCAGAAAGCCTATGTCCTGCATGCTGGAACAG(+AGGACA)GTACCTGTCTGTGTGTGGCCTTTCCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240613151716_rln4 CCCAGATGTGGGGAAAGGCCACACACAGACAG(+AGGACA)GTACCTGTTCCAGCATGCAGGACATAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613151714_uuno CCCATTTCCGTTCACAGTATGTTGGGGCCCAG(+AGGACA)GTACCAACAGTGTAGGGCTTTCTGATACCCT NGG 10 8 17 8 24 0 100 5 10